introduzione
The HiPure Mini system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Plasmid Mini Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Dettagli
Specifiche
| Caratteristiche | Specifiche |
| Funzioni principali | Isolation up to 70μg plasmid DNA from 5-15ml bacterial culture |
| Applicazioni | Enzyme digestion, sequenziamento, PCR, cloning, eccetera. |
| Metodo di purificazione | Mini colonna rotante |
| Tecnologia di purificazione | Tecnologia della silice |
| Metodo di processo | Manuale (centrifugazione o vuoto) |
| Tipo di campione | Conventional plasmid, plasmid less than 30KB |
| Importo del campione | High copy plasmid: 1-5ml culture medium Low copy plasmid : 5-10ml culture medium |
| Yield | 20-80mg |
| Volume di eluizione | ≥75μl |
| Tempo per corsa | Complete 1-24 samples in 30 minuti |
| Volume di trasporto del liquido per colonna | 800ml |
| Resa legante della colonna | 70mg |
Principio
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Vantaggi
- Purezza elevata – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, eccetera.
- Veloce – it takes only 2 minutes to obtain supernatant by optimized solution (10 minutes for other brands)
- High yield – up to 70µg plasmid can be binded in one column
Contenuto del kit
| Contenuti | P100202 | P100203 |
| Tempi di purificazione | 50 Preparativi | 250 Preparativi |
| RNasi A | 5 mg | 20 mg |
| Buffer P1 | 30 ml | 140 ml |
| Buffer P2 | 30 ml | 140 ml |
| Buffer P3 | 40 ml | 200 ml |
| Buffer PW1 | 30 ml | 140 ml |
| Buffer PW2* | 12 ml | 50 ml |
| Tampone di eluizione | 15 ml | 30 ml |
| HiPure DNA Mini Columns III | 50 | 250 ml |
| 2 ml Provette di raccolta | 50 | 250 ml |
Conservazione e stabilità
The kit components can be stored dry at room temperature (15-25°C) e sono stabili almeno per 18 mesi in queste condizioni. Se si formano dei precipitati nei tamponi, caldo a 37 ℃ per dissolversi. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.
Guida all'acquisto
| Nome | GATTO N | Importo del campione | Column type | Elutio volume | Yield | Tempo per corsa | Centrifuge requirements |
| Mini kit plasmide HiPure | P1001 | 1-5ml | 1.5ml column | 30-50ml | 5–35µg/5ml | ≤30 minutes | small centrifuge |
| HiPure Plasmid Mini Kit II | P1002 | 5-15ml | 1.5ml column | 75-100ml | 20-80µg/15ml | ≤30 minutes | small centrifuge |
| HiPure Plasmid Plus 96 Kit | P1006 | 1-1.5ml | 96 bene piatto | 70-150ml | 1-15μg/1ml | ≤60 minuti | 96 well plate centrifuge |
| HiPure Fastfilter Plasmid Midi Kit | P1013 | 30-50ml | 15ml column | 0.3-0.8ml | 50–250µg/50ml | ≤60 minuti | 15ml centrifuge |
| HiPure Fastfilter Plasmid Maxi Kit | P1014 | 100-200ml | 50ml column | 0.5-1.5ml | 0.4–1mg/200ml | ≤60 minuti | 50ml centrifuge |
Recensioni
Non ci sono ancora recensioni.