introduzione
- HiPure DNA plasmidico Più 96 Kit:
- Enables up to 96 mini-preps to be performed simultaneously.
- Process completed in less than 45 minutes using the Vacuum Manifold (Qiavac 96).
- Provides a fast, simple, and cost-effective method for high-throughput plasmid DNA extraction in routine molecular biology laboratory applications.
- Caratteristiche principali:
- Utilizes silica-membrane technology, eliminating cumbersome steps associated with loose resins or slurries.
- Plasmid DNA purified with the kit is immediately ready for use.
- Eliminates the need for phenol extraction and ethanol precipitation.
- High-quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Dettagli
Specifiche
| Caratteristiche | Specifiche |
| Funzioni principali | Isolation up to 20µg plasmid DNA from 1.5ml bacterial culture using 96 well bind plate and 96 filterplate |
| Applicazioni | Enzyme digestion, sequenziamento, PCR, labeling, eccetera. |
| Metodo di purificazione | 96 bene piatto |
| Tecnologia di purificazione | Tecnologia della silice |
| Metodo di processo | Manuale (centrifugazione o vuoto) |
| Tipo di campione | Conventional plasmid, plasmid less than 30KB |
| Importo del campione | 1-1.5ml（x96） |
| Yield | 1-15µg/1ml |
| Volume di eluizione | ≥70μl |
| Tempo per corsa | ≤60 minuti |
| Volume di trasporto del liquido per colonna | 800ml |
| Resa legante della colonna | 70mg |
Principio
- HiPure Plasmid Procedure:
- Based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt.
- Utilizes a unique silica membrane that replaces glass or silica slurries for plasmid DNA minipreps.
- Procedure Steps:
- Preparation and Clearing of Bacterial Lysate:
- The alkaline method is used to prepare and clear bacterial lysate.
- DNA Binding:
- Supernatant transferred to a column to bind DNA to the silica membrane.
- Washing and Elution:
- Proteins and other impurities washed away.
- Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
- Preparation and Clearing of Bacterial Lysate:
Vantaggi
- Purezza elevata – purified plasmid can be directly used in sequencing, enzyme digestion PCR, eccetera.
- Veloce – it takes only 60 minutes to complete the isolation
- High yield – up to 1mg plasmid can be bound in one column
- Economy – high cost-performance
Contenuto del kit
| Contenuti | P100601 | P100602 | P100603 |
| Tempi di purificazione | 1 X 96 Preparativi | 4 X 96 Preparativi | 20 X 96 Preparativi |
| RNasi A | 5 mg | 20 mg | 100 mg |
| Buffer P1 | 30 ml | 120 ml | 600 ml |
| Buffer P2 | 30 ml | 120 ml | 600 ml |
| Buffer P3 | 40 ml | 180 ml | 800 ml |
| Buffer PW1 | 100 ml | 500 ml | 2 X 1000 ml |
| Buffer PW2 | 50 ml | 2 X 100 ml | 4 X 200 ml |
| Tampone di eluizione | 150 ml | 60 ml | 300 ml |
| Lysate Clear Plate | 1 | 4 | 20 |
| Piastra DNA HiPure | 1 | 4 | 20 |
| 1.6 Piastra di raccolta ml | 1 | 4 | 20 |
| 0.5ml Elute Plate | 1 | 4 | 20 |
Conservazione e stabilità
The kit components can be stored dry at room temperature (15-25°C) e sono stabili almeno per 18 mesi in queste condizioni. Se si formano dei precipitati nei tamponi, caldo a 37 ℃ per dissolversi. After the addition of RNase A， Buffer P1 is stable for 6 months when stored at 2-8°C.
Guida all'acquisto
| Nome | GATTO N | Importo del campione | Column type | Volume di eluizione | Yield | Tempo per corsa | Centrifuge requirements |
| Mini kit plasmide HiPure | P1001 | 1-5ml | 1.5ml column | 30-50ml | 5–35µg/5ml | ≤30 minutes | small centrifuge |
| HiPure Plasmid Mini Kit II | P1002 | 5-15ml | 1.5ml column | 75-100ml | 20-80µg/15ml | ≤30 minutes | small centrifuge |
| HiPure Plasmid Plus 96 Kit | P1006 | 1-1.5ml | 96 bene piatto | 70-150ml | 1-15μg/1ml | ≤60 minuti | 96-well plate centrifuge |
| HiPure Fastfilter Plasmid Midi Kit | P1013 | 30-50ml | 15ml column | 0.3-0.8ml | 50–250µg/50ml | ≤60 minuti | 15ml centrifuge |
| HiPure Fastfilter Plasmid Maxi Kit | P1014 | 100-200ml | 50ml column | 0.5-1.5ml | 0.4–1mg/200ml | ≤60 minuti | 50ml centrifuge |
Recensioni
Non ci sono ancora recensioni.