Panoramica del Prodotto
- Offers fast and easy methods for purification of total DNA.
- Ensures reliable PCR and Southern blotting.
- Capable of purifying various types of DNA, including genomic, viral, and mitochondrial.
- Suitable for purification from tissue and cultured cells.
Specifiche
| Caratteristiche | Specifiche |
| Funzioni principali | Isolation of total DNA from <25 mg tissue, culture cells, FTA card |
| Applicazioni | PCR, Southern Bolt virus detection, eccetera. |
| Metodo di purificazione | Mini colonna rotante |
| Tecnologia di purificazione | Tecnologia della silice |
| Metodo di processo | Manuale (centrifugazione o vuoto) |
| Tipo di campione | Animal tissue or cultured cells |
| Importo del campione | Animal tissue : <25mg, Cultured cells : <5 X 106 |
| Volume di eluizione | ≥30μl |
| Tempo per corsa | 30 – 60 minuti |
| Volume di trasporto del liquido per colonna | 800ml |
| Resa legante della colonna | 100μg |
Principio
- Utilizes silica column purification method.
- The sample is lysed and digested with lysate and protease, releasing DNA into the lysate.
- Lysate is transferred to an adsorption column.
- Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration.
- Proteins and other impurities are washed away.
- Nucleic acid is finally eluted with low-salt buffer (10mm Tris, pH 9.0, 0.5mm EDTA).
Vantaggi
- Good repeatability – suitable for extracting high-yield DNA from different types of tissue samples
- Purezza elevata – can be used for downstream applications such as multiplex and quantitative PCR
- Fas t- simplified process, extracting several samples in 20 minuti (dopo la digestione)
- Safety – no phenol or chloroform extraction, no alcohol precipitation
Contenuto del kit
| Contenuti | D312102 | D312103 |
| Tempi di purificazione | 50 Preparativi | 250 Preparativi |
| ATL tampone | 15 ml | 65 ml |
| Buffer DL | 15 ml | 65 ml |
| Buffer GW1 | 22 ml | 110 ml |
| Buffer GW2 | 12 ml | 50 ml |
| RNasi A | 10 mg | 50 mg |
| Proteinasi K | 24 mg | 120 mg |
| Tampone di dissoluzione della proteasi | 5 ml | 15 ml |
| Buffer AE | 15 ml | 60 ml |
| HiPure gDNA Mini Columns | 50 | 2 X 125 |
| 2 ml Provette di raccolta | 100 | 5 X 100 |
Conservazione e stabilità
- All'arrivo, la RNasi A e la Proteinasi K devono essere conservate a 2-8°C.
- Short-term storage at room temperature (15-25°C) for up to 24 weeks does not affect their performance.
- Remaining kit components can be stored dry at room temperature (15-25°C) e sono stabili almeno per 18 mesi in queste condizioni.
- Alternatively, the entire kit can be stored at 2-8°C, but buffers should be redissolved before use.
- Ensure all buffers are at room temperature when used.
Recensioni
Non ci sono ancora recensioni.