- Challenges in DNA Extraction from Blood Samples:
- High Infectious Risk: Blood samples pose a significant infectious hazard to operators and the environment.
- Complex DNA Source: Blood DNA contains various types such as mitochondrial, genomic, and circulating DNA, leading to potential loss during extraction.
- Presence of Impurities: Blood samples contain impurities and inhibitory factors that can interfere with DNA extraction and downstream applications.
- Existing Extraction Methods:
- Phenol-chloroform extraction, salting out, eccetera.
- These methods typically involve pre-treatment steps to remove red blood cells and isolate white blood cells, which may result in loss of nucleic acid information.
- Product Benefits:
- Fast and Easy Purification: Provides a rapid and straightforward method for total DNA purification.
- Suitability: Purifies total DNA (genomic, viral, mitochondrial) from whole blood, tessuto, and cultured cells.
- Applicazione: Ensures reliable DNA for PCR and Southern blotting assays.
Specifiche
| Caratteristiche | Specifiche |
| Funzioni principali | Isolation of total DNA from 10ml blood and 1g tissue using Maxi column |
| Applicazioni | PCR, southern bolt and virus detection, eccetera |
| Metodo di purificazione | Maxi colonna rotante |
| Tecnologia di purificazione | Tecnologia della silice |
| Metodo di processo | Manuale (centrifugazione o vuoto) |
| Tipo di campione | Tessuto, cellule, sangue, saliva, swabs, blood spots, semen, and other clinical samples |
| Importo del campione | 3-10ml |
| Volume di eluizione | ≥700μl |
| Tempo per corsa | ≤90 minutes |
| Volume di trasporto del liquido per colonna | 4ml |
| Resa legante della colonna | 5mg |
Principio
- Cell Breakdown: Your sample is lysed (broken open) and digested with enzymes to release DNA into solution.
- Capture: The mixture is passed through a silica column. DNA sticks to the silica membrane, while proteins and other impurities don’t.
- Washing Away Impurities: The column is washed to remove any remaining proteins and other unwanted molecules.
- DNA Release: Pure DNA is eluted (washed off) the silica membrane with a special buffer solution. This purified DNA is then ready for further analysis.
Vantaggi
- High-quality DNA – meets a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, eccetera.
- Veloce – without separation of leukocytes, organic extraction, or ethanol precipitation
- Simple – all nucleic acids can be obtained by direct digestion
- Pertinence – specially designed for isolating DNA from 3-10ml blood and related body fluids
- Wide applicability – handle a variety of liquid samples
- Economy – excellent cost-effectiveness performance
Contenuto del kit
| Contents10 | 10 preparazioni | 50 perps |
| Tempi di purificazione | 10 | 50 |
| HiPure gDNA Maxi Columns | 10 | 50 |
| 50ml Provette di raccolta | 20 | 100 |
| ATL tampone | 120 ml | 550 ml |
| Buffer AL | 120 ml | 2 X 300 ml |
| Buffer GW1* | 53 ml | 220 ml |
| Buffer GW2* | 25 ml | 110 ml |
| RNasi A | 40 mg | 180 ml |
| Proteinasi K | 120 mg | 540 mg |
| Tampone di dissoluzione della proteasi | 12 ml | 50 ml |
| Buffer AE | 30 ml | 120 ml |
Conservazione e stabilità
Storage Recommendations:
- Proteinase K and RNase A: Upon arrival, store at 2-8°C. Short-term storage (fino a 12 settimane) a temperatura ambiente (15-25°C) is acceptable without affecting performance.
- Remaining Kit Components: Can be stored at room temperature (15-25°C) and remain stable for at least 18 mesi in queste condizioni.
Guida all'acquisto
| Nome | GATTO N | Importo del campione | Leukocyte protocol* | Colum type | Volume di eluizione | Average yield | Tempo per corsa |
| Mini kit HiPure per il DNA del sangue | D3111 | 10-200ml | 1ml | 2ml column | ≥20μl | 5-9μg/200μl | ≤30 minutes |
| Tessuto HiPure&Blood DNA Midi Kit | D3113 | 0.2-2ml | 10ml | 1.5ml column | ≥300μl | 20-40μg/1m | ≤80 minutes |
| Tessuto HiPure&Blood DNA Maxi Kit | D3115 | 3 -10ml | 10ml | 15ml column | ≥700μl | 20-40μg/1m | ≤90 minutes |
| Tessuto HiPure&DNA del sangue 96 Kit | D3117 | 1-200ml | 1ml | 96 bene piatto | 3-8μg/200μl |
