Panoramica del Prodotto
- Offers fast and easy purification methods for total DNA.
- Ensures reliable PCR and Southern Blotting results.
- Can purify various types of DNA: genomic, viral, and mitochondrial.
- Suitable for purification from whole blood, tessuto, and cultured cells.
Specifiche
| Caratteristiche | Specifiche |
| Funzioni principali | Isolation of total DNA from 10ml blood and 1g tissue using Maxi column |
| Applicazioni | PCR, southern bolt and virus detection, eccetera |
| Metodo di purificazione | Maxi colonna rotante |
| Tecnologia di purificazione | Tecnologia della silice |
| Metodo di processo | Manuale (centrifugazione o vuoto) |
| Tipo di campione | Tessuto, cellule, sangue, saliva, swabs, blood spots, semen, and other clinical samples |
| Importo del campione | 3-10ml |
| Volume di eluizione | ≥700μl |
| Tempo per corsa | ≤90 minutes |
| Volume di trasporto del liquido per colonna | 4ml |
| Resa legante della colonna | 5mg |
Principio
- Utilizes silica column purification method.
- The sample is lysed and digested with lysate and protease, releasing DNA into the lysate.
- Lysate is transferred to an adsorption column.
- Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed through filtration.
- Proteins and other impurities are washed away.
- Nucleic acid is finally eluted with low-salt buffer (10mm Tris, pH 9.0, 0.5mm EDTA).
Vantaggi
- High-quality DNA – meets a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, eccetera.
- Veloce – without separation of leukocytes, organic extraction, or ethanol precipitation
- Simple – all nucleic acids can be obtained by direct digestion
- Pertinence – specially designed for isolating DNA from 3-10ml blood and related body fluids
- Wide applicability – handle a variety of liquid samples
- Economy – excellent cost-effectiveness performance
Contenuto del kit
| Contenuti | D311502 | D311503 |
| Tempi di purificazione | 10 | 50 |
| HiPure gDNA Maxi Columns | 10 | 50 |
| 50ml Provette di raccolta | 20 | 100 |
| ATL tampone | 120 ml | 550 ml |
| Buffer AL | 120 ml | 2 X 300 ml |
| Buffer GW1* | 53 ml | 220 ml |
| Buffer GW2* | 25 ml | 110 ml |
| RNasi A | 40 mg | 180 ml |
| Proteinasi K | 120 mg | 540 mg |
| Tampone di dissoluzione della proteasi | 12 ml | 50 ml |
| Buffer AE | 30 ml | 120 ml |
Conservazione e stabilità
- Proteinase K and RNase A should be stored at 2-8°C upon arrival.
- Short-term storage at room temperature (15-25°C) for up to 12 weeks does not affect their performance.
- I restanti componenti del kit possono essere conservati a temperatura ambiente (15-25°C) and remain stable for at least 18 mesi in queste condizioni.
Guida all'acquisto
| Nome | GATTO N | Importo del campione | Leukocyte protocol* | Colum type | Volume di eluizione | Average yield | Tempo per corsa |
| Mini kit HiPure per il DNA del sangue | D3111 | 10-200ml | 1ml | 2ml column | ≥20μl | 5-9μg/200μl | ≤30 minutes |
| Tessuto HiPure&Blood DNA Midi Kit | D3113 | 0.2-2ml | 10ml | 1.5ml column | ≥300μl | 20-40μg/1m | ≤80 minutes |
| Tessuto HiPure&Blood DNA Maxi Kit | D3115 | 3 -10ml | 10ml | 15ml column | ≥700μl | 20-40μg/1m | ≤90 minutes |
| Tessuto HiPure&DNA del sangue 96 Kit | D3117 | 1-200ml | 1ml | 96 bene piatto | 3-8μg/200μl |
Recensioni
Non ci sono ancora recensioni.