Blood is a valuable source of DNA for clinical diagnostics and research, but traditional extraction methods present challenges:
- Safety Hazards: Bloodborne pathogens pose a risk of infection during extraction.
- DNA Loss: Red blood cell removal can eliminate desired DNA fractions (viral, circulating DNA).
- Impurities and Inhibitors: Blood components can interfere with downstream applications (PCR, Southernblotting).
This product offers a novel DNA extraction solution that addresses these issues:
- Safe and Efficient: No red blood cell removal minimizes infectious waste and contamination risks.
- Comprehensive DNA Recovery: Purifies total DNA (genomic, mitochondrial, viral, circulating) for a complete picture.
- Broad Sample Compatibility: Works with whole blood (fresh/frozen), plasma, siero, cappotto buffy, and cultured cells.
- Downstream Applications: Purified DNA is suitable for PCR and Southern blotting.
This innovative method simplifies blood DNA extraction while ensuring safety, efficiency, and comprehensive DNA recovery. It represents a significant advancement for researchers in diagnostics, forensics, and medical research.
Nota: This bulletin corrects the previous statement about sample compatibility. This method is not suitable for tissue samples.
Specifiche
| Caratteristiche | Specifiche |
| Funzioni principali | Isolation of total DNA from 2ml blood and 200mg tissue using Midi column |
| Applicazioni | PCR, southern bolt and virus detection, eccetera |
| Metodo di purificazione | Midi colonna rotante |
| Tecnologia di purificazione | Tecnologia della silice |
| Metodo di processo | Manuale (centrifugazione o vuoto) |
| Tipo di campione | Tessuto, cellule, sangue, saliva, swabs, blood spot, semen, and other clinical samples |
| Importo del campione | 0.2-2 ml |
| Volume di eluizione | ≥300μl |
| Tempo per corsa | ≤80 minutes |
| Volume di trasporto del liquido per colonna | 4ml |
| Resa legante della colonna | 1mg |
Principles
This product offers a streamlined method for purifying DNA from blood samples using silica column technology.
The Process:
- Lysis and Digestion: The blood sample is treated with a lysate solution and protease to break open cells and degrade proteins, releasing DNA into the solution.
- Binding to Silica: The lysate is transferred to a silica column. The silica membrane in the column selectively binds DNA molecules, while proteins and other impurities pass through.
- Washing: Wash buffers remove unbound proteins and cellular debris that didn’t adhere to the silica.
- Elution: A low-salt buffer (typically 10 mM Tris, pH 9.0, E 0.5 mM EDTA) is used to elute the purified DNA from the silica. This disrupts the interaction between DNA and the silica, allowing the collection of purified DNA in a separate tube.
Vantaggi
- High-quality DNA – meets a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, eccetera.
- Veloce – without separation of leukocytes, organic extraction, or ethanol precipitation
- Simple – all nucleic acids can be obtained by direct digestion
- Wide applicability – handle a variety of liquid samples
Contenuto del kit
| Contenuti | D311302 | D311303 |
| Tempi di purificazione | 20 | 100 |
| HiPure gDNA Midi Columns | 20 | 100 |
| 15ml Provette di raccolta | 40 | 200 |
| ATL tampone | 50 ml | 250 ml |
| Buffer AL | 50 ml | 250 ml |
| Buffer GW1* | 22 ml | 110 ml |
| Buffer GW2* | 12 ml | 50 ml |
| RNasi A | 20 mg | 90 mg |
| Proteinasi K | 100 mg | 440 mg |
| Tampone di dissoluzione della proteasi | 10 ml | 30 ml |
| Buffer AE | 20 ml | 120 ml |
Conservazione e stabilità
For optimal performance:
- Store Proteinase K and RNase A at 2-8°C (refrigerator) upon arrival.
Acceptable short-term storage:
- Proteinase K and RNase A can be stored for up to 12 weeks at room temperature (15-25°C) without affecting their performance.
Remaining kit components:
- Store all other components of the kit dry at room temperature (15-25°C).
- They are stable for at least 18 mesi in queste condizioni.
Important Note:
- If storing the entire kit at room temperature, ensure buffers are redissolved before use and always warm all buffers to room temperature before using them.
Guida all'acquisto
| Nome | GATTO N | Importo del campione | Leukocyte protocol* | Colum type | Volume di eluizione | Average yield | Tempo per corsa |
| Mini kit HiPure per il DNA del sangue | D3111 | 10-200ml | 1ml | 2ml column | ≥20μl | 5-9μg/200μl | ≤30 minutes |
| Tessuto HiPure&Blood DNA Midi Kit | D3113 | 0.2-2ml | 10ml | 1.5ml column | ≥300μl | 20-40μg/1m | ≤80 minutes |
| Tessuto HiPure&Blood DNA Maxi Kit | D3115 | 3 -10ml | 10ml | 15ml column | ≥700μl | 20-40μg/1m | ≤90 minutes |
| Tessuto HiPure&DNA del sangue 96 Kit | D3117 | 1-200ml | 1ml | 96 bene piatto | 3-8μg/200μl |
