Panoramica del Prodotto
- Suitable for rapid extraction of total DNA from various sources: tessuto, cellule, sangue, saliva, swabs, blood spots, semen, and other clinical samples.
- Extracted DNA can be used directly for PCR, quantitative PCR, Southern Blot, virus DNA testing, e altre applicazioni.
Specifiche
| Caratteristiche | Specifiche |
| Funzioni principali | Isolation of total DNA from tissue/blood/body fluid/swab/dry blood spots |
| Applicazioni | PCR, qPCR, southern bolt and virus detection, eccetera |
| Metodo di purificazione | Mini colonna rotante |
| Tecnologia di purificazione | Tecnologia della silice |
| Metodo di processo | Manuale (centrifugazione o vuoto) |
| Tipo di campione | Tessuto, cellule, sangue, saliva, swabs, blood spots, semen, and other clinical samples |
| Importo del campione | Solid tissue: 1-10mg; Anticoagulant blood: 200ml |
| Volume di eluizione | ≥20μl |
| Tempo per corsa | 30 – 60 minuti |
| Volume di trasporto del liquido per colonna | 800ml |
| Resa legante della colonna | 100mg |
Principio
- Utilizes silica column purification method.
- The sample is lysed and digested with lysate and protease, releasing DNA into the lysate.
- Lysate is transferred to an adsorption column.
- Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and removed through filtration.
- Proteins and impurities are washed away.
- Nucleic acid is finally eluted with low-salt buffer (10mm Tris, pH 9.0, 0.5mm EDTA).
Vantaggi
- High-quality DNA – meets a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, eccetera.
- Veloce – without separation of leukocytes, organic extraction, or ethanol precipitation
- Simple – all nucleic acids can be obtained by direct digestion
- Wide applicability- handle a variety of liquid samples
Contenuto del kit
| Contenuti | D301802 | D301803 |
| Tempi di purificazione | 50 | 250 |
| HiPure DNA Mini Columns I | 50 | 250 |
| 2ml Provette di raccolta | 100 | 500 |
| ATL tampone | 30 ml | 150 ml |
| Buffer AL | 30 ml | 150 ml |
| Buffer GW1 | 22 ml | 88 ml |
| Buffer GW2 | 12 ml | 50 ml |
| Proteinasi K | 24 mg | 120 mg |
| Tampone di dissoluzione della proteasi | 1.8 ml | 10 ml |
| Buffer AE | 15 ml | 60 ml |
Conservazione e stabilità
- Proteinase K should initially be stored at 2-8°C upon arrival.
- Short-term storage at room temperature (15-25°C) for up to 12 weeks does not affect its performance.
- I restanti componenti del kit possono essere conservati a temperatura ambiente (15-25°C) and remain stable for at least 18 mesi.
- Alternatively, the entire kit can be stored at 2-8°C, but buffers should be redissolved before use.
- Ensure all buffers are at room temperature when used.
Dati dell'esperimento
Recensioni
Non ci sono ancora recensioni.