This product provides fast and easy methods for the purification of total DNA for reliable PCR and southern blotting. Total DNA (per esempio., genomic, plasmid) can be purified from bacterial cultures.
Specifiche
| Caratteristiche | Specifiche |
| Funzioni principali | Isolation total DNA from bacterial culture |
| Applicazioni | PCR, Southern blot e digestione enzimatica, eccetera. |
| Tecnologia di purificazione | Tecnologia delle perle magnetiche |
| Metodo di processo | Manuale o automatico |
| Tipo di campione | Gram negative or positive bacteria |
| Importo del campione | Bacterial culture: 0.5-1.5ml |
| Volume di eluizione | ≥60μl |
| Tempo per corsa | ≤60 minuti |
Principio
- This product utilizes high-binding magnetic particles for purification.
- Samples are lysed and digested using a lysate and Lysozyme.
- DNA is released into the lysate during this process.
- Magnetic particles and binding solution are added, allowing DNA to be adsorbed onto the surface of the particles while impurities such as proteins are not adsorbed.
- The adsorbed particles undergo washing with a washing solution to remove proteins and impurities.
- Ethanol is used for further washing to remove salts.
- Finalmente, DNA is eluted using Elution Buffer.
Vantaggi
- Veloce: Multiple samples can be extracted within 60 minuti (dopo la digestione), making the process time-efficient.
- Purezza elevata: Unique adsorbents ensure the complete removal of inhibitory factors, resulting in high-purity DNA extraction.
- Recupero elevato: DNA can be recovered at the level of picograms (PG), indicating a high level of efficiency in the extraction process.
Contenuto del kit
| Contenuti | D513101 | D513102 | D513103 |
| Tempi di purificazione | 48 Preparativi | 96 Preparativi | 480 Preparativi |
| Particelle MagPure | 1.2 ml | 2.5 ml | 11 ml |
| Lysozyme | 60 mg | 120 mg | 600 mg |
| Proteinasi K | 24 mg | 50 mg | 220 mg |
| Tampone di dissoluzione della proteasi | 3 ml | 6 ml | 30 ml |
| Respingente Scheda di sicurezza | 1.5 ml | 3 ml | 15 ml |
| Buffer MLA | 30 ml | 60 ml | 300 ml |
| Buffer GW1* | 13 ml | 44 ml | 110 ml |
| Buffer TE | 30 ml | 40 ml | 200 ml |
Conservazione e stabilità
- Particelle MagPure, Lysozyme, and Proteinase K should be stored at 2-8°C upon arrival.
- Short-term storage (fino a 12 settimane) a temperatura ambiente (15-25°C) non influisce sulle loro prestazioni.
- I restanti componenti del kit possono essere conservati asciutti a temperatura ambiente (15-25°C) and remain stable for at least 18 mesi in queste condizioni.
Recensioni
Non ci sono ancora recensioni.