introduzione
- Extracts DNA from various sources: tessuto, cellule, sangue, saliva, swabs, blood spots, semen, and other clinical samples.
- Fast and easy to use.
- Purified DNA can be used for PCR, qPCR, Southern Blot, viral DNA testing, e altro ancora.
Specifiche
| Caratteristiche | Specifiche |
| Funzioni principali | Isolation of total DNA from 1-100ul blood, FFPE, tessuto, and other samples |
| Applicazioni | Second-generation sequencing, PCR, PCR in tempo reale, eccetera. |
| Metodo di purificazione | Monodisperse magnetic beads |
| Tecnologia di purificazione | Tecnologia delle perle magnetiche |
| Metodo di processo | Manuale o automatico |
| Tipo di campione | Anticoagulant blood, concentrated blood, cappotto buffy, lymphocytes, and cultured cells |
| Importo del campione | Body fluid : 10 – 200ml, Tessuto : ≤10mg |
| Yield | 10ng – 15μg |
| Volume di eluizione | 80 -100ml |
| Tempo per corsa |
Principio
- Utilizes high-binding magnetic particles for purification, ensuring efficient DNA isolation.
- The sample undergoes lysis and digestion facilitated by lysate and Protease, releasing DNA into the solution.
- Magnetic particles and binding solution are introduced, causing DNA to adhere to particle surfaces while impurities such as proteins are left unbound.
- Wash with a specialized solution to remove proteins and impurities, followed by ethanol wash to eliminate salts.
- DNA is ultimately eluted using an Elution Buffer, providing purified DNA ready for downstream applications.
Advantage
- High binding capability – ideal for processing DNA-rich samples like whole blood, cappotto buffy, and concentrated blood.
- Exceptional purity – features carboxyl magnetic beads with minimal surface adsorption, ensuring superior purity.
- Versatile – applicable across a wide range of clinical samples, offering general utility.
Contenuto del kit
| Contenuti | IVD3101 |
| Tempi di purificazione | 200 |
| Legare le particelle | 4.5 ml |
| Proteinasi K | 90 mg |
| Tampone di dissoluzione della proteasi | 10 ml |
| ATL tampone | 60 ml |
| Buffer AL | 60 ml |
| Buffer BD* | 20 ml |
| Buffer BXW1* | 110 ml |
| Tampone di eluizione | 30 ml |
Conservazione e stabilità
- Proteinase K and MagBind Particles should be stored at 2–8°C upon receipt.
- Short-term storage (fino a 18 settimane) a temperatura ambiente (15–25°C) does not compromise their performance.
- The remaining components can be stored dry at room temperature (15–25°C) and remain stable for at least 18 mesi in queste condizioni.
