Fast and Easy Total Purificazione del DNA
- Objective: Reliable PCR and Southern Blotting
- Caratteristiche:
- Swift purification methods
- Easy-to-follow protocols
- Benefici:
- Saves time and effort
- Ensures reliable PCR and Southern blotting results
- Applicability:
- Purifies various types of total DNA:
- Genomic
- Viral
- Mitochondrial
- Suitable for diverse sample types:
- Sangue intero
- Plasma
- Siero
- Buffy coat
- Bone marrow
- Other body fluids
- Lymphocytes
- Cultured cells
- Purifies various types of total DNA:
Specifiche
| Caratteristiche | Specifiche |
| Funzioni principali | Isolation of total DNA from 200μl whole blood |
| Applicazioni | PCR, southern bolt and virus detection, eccetera |
| Tecnologia di purificazione | Tecnologia delle perle magnetiche |
| Metodo di processo | Manuale o automatico |
| Tipo di campione | Sangue intero, concentrated blood, cappotto buffy, and other samples |
| Importo del campione | 200ml |
| Volume di eluizione | ≥70μl |
| Tempo per corsa | ≤40 minutes |
Principio
- Sample lysis and digestion facilitated by lysate and Protease.
- Il DNA viene rilasciato nel lisato.
- DNA adsorption onto magnetic particles’ surface, excluding impurities like proteins.
Procedura:
- Sample lysis and digestion: Lysate and Protease act on the sample.
- DNA release: Il DNA viene rilasciato nel lisato.
- DNA adsorption: Magnetic particles and binding solution are added, facilitating DNA adsorption.
- Impurity removal: Proteins and impurities are removed without DNA adsorption.
- Washing:
- First, wash with washing solution to remove proteins and impurities.
- Ethanol wash to eliminate salts.
- Elution: DNA is eluted using an Elution Buffer.
Vantaggi:
- High specificity: Selective DNA binding, excluding impurities.
- Efficienza: Rapid purification process.
- Minimal sample loss: Enhanced DNA recovery.
Applicazioni:
- Ideal for PCR and Southern blotting experiments requiring purified DNA.
- Suitable for various sample types and DNA sources.
Vantaggi
- High Binding Force: Magnetic beads with exceptional binding capacity efficiently capture DNA molecules even from samples with abundant DNA, such as whole blood, cappotto buffy, and concentrated blood.
- Rapid Processing: The use of polydisperse magnetic beads ensures a fast magnetic response, leading to significantly reduced extraction times.
- High-Purity DNA: The optimized protocol delivers highly purified DNA, eliminating the need for further purification steps. This high-quality DNA is directly usable for various downstream applications:
- Second-generation sequencing (NGS)
- PCR-based detection methods
- Gene bank construction
- And other DNA analyses requiring high purity
Contenuto del kit
| Contenuti | D631001 | D631002 | D631003 |
| Tempi di purificazione | 48 | 96 | 480 |
| Particelle MagPure | 1.7 ml | 3.5 ml | 17 ml |
| Proteinasi K | 24 mg | 50 mg | 220 mg |
| Tampone di dissoluzione della proteasi | 1.8 ml | 3 ml | 15 ml |
| Buffer MLA | 30 ml | 60 ml | 270 ml |
| Buffer DW1 | 60 ml | 120 ml | 550 ml |
| Buffer CW | 30 ml | 60 ml | 270 ml |
| Buffer BW3 | 30 ml | 60 ml | 270 ml |
| Tampone di eluizione | 15 ml | 30 ml | 100 ml |
Conservazione e stabilità
Proteinasi K, MagPureParticles should be stored at 2-8°C upon arrival. Tuttavia, conservazione a breve termine(fino a 24 settimane) a temperatura ambiente (15-25°C) non influisce sulle loro prestazioni. I restanti componenti del kit possono essere conservati a temperatura ambiente (15-25°C)e sono stabili almeno per 18 mesi in queste condizioni.
