introduzione
This product is specially designed for stool Estrazione dell'RNA. The kit is suitable for extracting high-purity microbial or host cell RNA from ≤ 0.15g stool samples. The purified RNA can be directly used in RT-PCR and Northern hybridization.
Dettagli
Principio
The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.
Contenuto del kit
| Contenuti | R662601 | R662602 | R662603 |
| Tempi di purificazione | 48 Preparativi | 96 Preparativi | 5 X 96 Preparativi |
| MagPure RNA Particles | 1.7 ml | 4.0 ml | 18 ml |
| DNasi I | 600 ml | 2 X 600 ml | 10 X 600 ml |
| DNase Buffer | 30 ml | 40 ml | 200 ml |
| Buffer SPL | 30 ml | 60 ml | 270 ml |
| PHC tampone | 30 ml | 60 ml | 270 ml |
| Buffer MCB* | 18 ml | 30 ml | 150 ml |
| Buffer MW1* | 44 ml | 66 ml | 2 X 220 ml |
| Buffer RW2* | 20 ml | 50 ml | 2 X 100 ml |
| Acqua libera da RNasi | 10 ml | 30 ml | 120 ml |
| 2ml Tubi a perline | 48 | 96 | 5 X 96 |
Conservazione e stabilità
MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. Tuttavia, conservazione a breve termine (DNase I up to 1 settimane, MagPure RNA Particles up to 8 settimane) a temperatura ambiente (15–25°C) non influisce sulle loro prestazioni. I restanti componenti del kit possono essere conservati a temperatura ambiente (15–25°C) e sono stabili almeno per 18 months under these
Recensioni
Non ci sono ancora recensioni.