Name: Pre-processing of plant sample genomic DNA extraction 96 Samples
SKU: 200
Price: 225.00 USD
Availability: InStock

Pre-processing of plant sample genomic DNA extraction 96 Campioni

$225.00

Spedizione USD 45 - Gratuito per USD 300

DTE è una piattaforma di e-commerce con sede in Cina specializzata nella vendita online di test molecolari, ELISA, e prodotti correlati.

Gestione dei campioni:
- UN. Material Collection & Magazzinaggio:
  - If collected fresh materials cannot be used immediately, cool them in liquid nitrogen and store them at -80°C. Dried materials can be stored in the refrigerator.
- B. Collection Preference:
  - Collect fresh material whenever possible, as it contains fewer polysaccharides and polyphenols.
- C. Fungal Sample Collection:
  - Fungi can be collected by centrifugation from liquid media.
Preparazione del campione:
- Grind about 100 mg of fresh samples or no more than 20 mg of dry matter with liquid nitrogen.
- Aggiungere 400 μl Buffer I and 10 µl di RNasiA (10 mg/ml) to ensure no lumpy tissue in the sample, which can reduce DNA yield.
- Incubate at 65°C for 2-10 minuti, invert, and mix 2-3 times during this period. Extend incubation time if the sample is difficult to lyse, but not more than 1 hour.
- Aggiungere 130 μl Buffer II, mescolare bene, and incubate on ice for 5 minutes to precipitate polysaccharides and proteins.
Purification:
- Add the lysate to the purification column and centrifuge at 13,000 giri al minuto per 2 minuti.
Transfer to Reagent Plate:
- Add the centrifuged supernatant from the previous step to the 96-well plate.
- Follow specific steps outlined in the automatic extraction protocol for further processing.

Peso

0.7 kg

marchio

Pre-processing of plant sample genomic DNA extraction 96 Campioni

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