導入
- Technology: Utilizes paramagnetic bead technology for rapid and high-performance dye-terminator removal.
- No Centrifugation or Filtration: Eliminates the need for centrifugation or filtration steps, simplifying the purification process.
- Manual or Automated: This can be performed manually or fully automated for high-throughput applications, providing flexibility in workflow.
- Improved Sequencing Results: Produces sequences with longer Phred 20 read lengths and higher signal intensities compared to similar purification systems.
- Enhanced Performance: Offers superior performance in terms of sequence quality and signal intensity, enhancing downstream analysis and interpretation.
仕様
特徴 | 仕様 |
主な機能 | Removal of free fluorescent dye from sequencing solution (Replace Beckmen or Agincourt CleanSeq) |
アプリケーション | Automated sequences |
精製技術 | 磁気ビーズ技術 |
加工方法 | 手動または自動 |
サンプルの種類 | DNA doped with fluorescent dye |
サンプル量 | 5μl |
回復 | 90% |
溶出量 | ≥25μl |
稼働時間 | 50分以内 |
原理
CleanSeq offers a rapid and efficient method for purifying sequencing reaction products, ideal for applications like Sanger sequencing.
- Magnetic Bead Technology: Utilizes magnetic particles within an optimized binding buffer to selectively capture desired DNA fragments.
- In-Plate Purification: The entire protocol can be performed directly in the thermal cycling plate, eliminating the need for transfer steps.
- Efficient Cleanup: Removes unincorporated dyes, ヌクレオチド, 塩, and contaminants through a simple washing procedure.
- Automation Friendly: No centrifugation or filtration is required, making CleanSeq compatible with various automated liquid handling platforms.
This streamlined approach minimizes processing time and simplifies workflow, making CleanSeq a valuable tool for high-throughput sequencing applications.
利点
- 高い回復力 – まで 90%
- 高スループット – 磁気ビーズ精製技術を使用
- Simple – simplified operation process, combination – washing – elution
キット内容
コンテンツ | BCS-5 | BCS-50 | BCS-500 |
クリーンシークビーズ | 5 ミリリットル | 50 ミリリットル | 500 ミリリットル |
保管と安定性
ストレージ:
- For optimal performance, store CleanSeq Beads at 2-8°C (refrigerator) upon arrival and up to 6 月.
- Short-term storage (まで 12 週) 室温で (15-25℃) is acceptable without affecting performance.
Before Use:
- Ensure proper mixing of CleanSeq Beads before each use.
- The reagent should appear homogenous and consistent in color throughout.
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