導入
本製品は、厳格な選別と品質管理を経た核酸抽出用の中核原料です。. Guanidine hydrochloride is a powerful denaturantor ionizing agent, which can promote the solubility of hydrophobic molecules and denature proteins. It is also an effective denaturant and inactivator of avariety of enzymes and proteins. In 6 M guanidine hydrochloride solution, the ordered “secondary structure” (maintained by intermolecular non covalent interactions, such as hydrogen bonds and hydrophobic bonds) of almost all proteins is destroyed and the peptide chain is stretched. The tertiary structure has changed. In the process of nucleic acid extraction, guanidine hydrochloride is often used as cleaning solution (> 3M), binding solution (4-6M) for protein removal, and lysate for genomic DNA and total RNA抽出. Different from guanidine isothiocyanate, guanidine hydrochloride has low absorbance in A260, A280 and A230, and the ratio of nucleic acid product A260 /230 is more ideal.
詳細
仕様
| 特徴 | 仕様 | |
| 基本的な内容 | 推奨アプリケーション | Plasmid extraction, lysis Solution or cleaning solution |
| CAS番号 | 50-01-1 | |
| 式 | CH5N3.HCL | |
| 分子量 | 95.53 | |
| Content | 99.5% | |
| レベル | 分子生物学 | |
| 外観 | ホワイトクリスタル, 長時間硬化します | |
| 輸送条件 | 室温 | |
| 保存条件 | 室温, ドライ, 暗い | |
| 不純物パラメータ | 水分 | ≤0.3% |
| Ash | ≤0.02% | |
| Water insoluble matter | ≤0.02% | |
| Ammonium | ≤0.05% | |
| Ammonium dicyanide | ≤0.1% | |
| Ammonium cyanuric chloride | ≤0.01% | |
| 鉄 | ≤0.1PPM | |
| 紫外線吸収値 | 吸光度@ 230 (6M) | ≤0.4 |
| 吸光度@ 260 (6M) | ≤0.04 | |
| 吸光度@ 280 (6M) | ≤0.01 | |
| 吸光度@ 320 (6M) | ≤0.01 | |
| 核酸抽出関連 | Plasmid extraction test | 採用する |
| Blood DNA extraction test | 採用する | |
| DNase テスト (2M) | 検出されず | |
| RNase検査 (2M) | 検出されず | |
| Purity of saturated solution (8M) | The 8m mother liquor is heated and dissolved. After dissolution, the insoluble matter is filtered with 3 layers of rapid filter paper. | |
| PH value (6M) | 5.0-5.5 |
注文情報
| カタログ番号. | 商品名 | パッケージ |
| C11101 | Guanidine Hydrochloride (分子生物学) | 5KG |
| C11102 | 25KG (5KG/Bag, 5Bags/Bucket) |
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