導入

HiPure Bacterial RNA Kit uses silica gel column purification to simplify the extraction. The whole process does not require phenol chloroform extraction and time-consuming alcohol precipitation. The kit is suitable for efficiently extracting RNA from various bacterial samples. The purified RNA can be directly used for RT-PCR, Northern hybridization, 等. The kit has included lysozyme and glass beads, which can be used to treat gram-negative bacteria which is easy to be lysed, as well as gram-positive bacteria which is hard to be lysed, including enterococcus faecalis, staphylococcus, 等.

詳細

仕様

原理

Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as グアニジン hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

利点

• 速い – several samples can be extracted in 30 分
• 高純度 – the purified RNA can be directly used in various downstream applications
• 高い回復力 – RNA can be recovered at the level of PG
• Good repeatability – silica gel column purification technology can obtain ideal results every time
• Broad spectrum – it can deal with various bacteria, including Gram-positive bacteria that are difficult to be lysed
• Sufficient components – the kit contains carried lysozyme and glass beads

キット内容

保管と安定性

MagZol Reagent and Lysozyme should be stored at 2-8°C upon arrival. 残りのキットコンポーネントは乾燥した状態で室温で保管できます。 (15-25℃) 少なくとも一定期間は安定しています 18 この状況下で数か月. キット全体は 2 ～ 8°C で保存できます。, ただし、この場合、バッファーは使用前に再溶解する必要があります。. 使用するときはすべてのバッファーが室温であることを確認してください.