HiPure Blood RNA ミニキット

The Kit provides fast purification of high-quality RNA from whole blood, 細胞, and tissues using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. The purified RNA can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

詳細

仕様

原理

The Kit simplifies the isolation of RNA from blood with a fast spin-column procedure. Red blood cells are selectively lysed and white cells are collected by centrifugation. White cells are then lysed using highly denaturing conditions which immediately inactivate RNases. After homogenization using the DNA spin column, the sample is applied to the RNA column. Total RNA binds to the membrane and contaminants are washed away, leaving pure RNA to be eluted in 30–100µl RNase-free water (provided with the kit) for direct use in any downstream application.

利点

• High quality – high purity total RNA can be directly used in various sensitive downstream applications
• 速い – isolation of several samples can be completed in 20 minutes by using the column purification method
• Sensitive – RNA can be purified at the level of PG

キット内容

保管と安定性

HiPure Blood RNA Mini Kit components can be stored dry at room temperature (15-25℃) 少なくとも一定期間は安定しています 18 この状況下で数か月. 使用するときはすべてのバッファーが室温であることを確認してください. During shipment, crystals or precipitation may form in the RTL Lysis Buffer. Dissolve by warming buffer to 37°C.