導入
The Kit provides fast purification of high-quality DNA, RNA and Prote from cells, 組織, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated. RNA purified using the HiPure Total RNA Purification System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
詳細
仕様
| 特徴 | 仕様 |
| 主な機能 | Co-isolation DNA and RNA /protein from a single sample (細胞, soft tissue, plant sample) |
| アプリケーション | SDS-PAGE electrophoresis and western blot, 等 |
| 精製方法 | Mini スピンカラム |
| 精製技術 | シリカ技術 |
| 加工方法 | マニュアル (遠心分離または真空) |
| サンプルの種類 | Culture cells, animal tissues, plant fungi, yeast, bacteria and other samples |
| サンプル量 | Cultured cells: < 10^7 Animal tissue: ≤ 20 mg Plant samples: ≤ 150 mg Yeast cells: 2 バツ 10^6 – 5 バツ 10^7 |
原理
The Kit isolates total RNA from up to 10 7 cells or 30 mg tissue. A short workflow enables RNAisolation with genomic DNA removal in less than 25 分. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30 µl water using the Kit.
利点
- High quality – high purity total RNA / DNA can be directly used in a variety of downstream applications
- 速い – column method can complete the extraction of several samples in 30 分
- Safe – no phenol chloroform extraction required
- Simultaneous extraction- simultaneously isolate DNA and RNA from one sample
キット内容
| コンテンツ | R521102 | R521103 |
| 浄化時間 | 50 準備 | 250 準備 |
| HiPure DNA Mini Columns | 50 | 250 |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml 採取管 | 100 | 2 バツ 250 |
| Buffer RLC | 50 ミリリットル | 200 ミリリットル |
| バッファGW1* | 22 ミリリットル | 66 ミリリットル |
| Buffer RW1 | 50 ミリリットル | 200 ミリリットル |
| Buffer RW2* | 50 ミリリットル | 3 バツ 50 ミリリットル |
| RNase Free Water | 10 ミリリットル | 30 ミリリットル |
| 溶出バッファー | 10 ミリリットル | 30 ミリリットル |
| Buffer ALO(5%SDS) | 10 ミリリットル | 30 ミリリットル |
保管と安定性
HiPure Kit can be stored dry at room temperature (15–25℃) 少なくとも一定期間は安定しています 18 この状況下で数か月. During shipment, crystals or precipitation may form in the Buffer RLC. Dissolve by warming buffer to 37°C.
レビュー
まだレビューはありません.