The HiPure Fruit RNA Mini Kit provides fast purification of high-quality RNA from fruits, 細胞, 組織, and yeast using silica-membrane spin columns with a binding capacity of 100µg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA(mRNA) purification, nuclease protection and in vitro translation.
詳細
仕様
| 特徴 | 仕様 |
| 主な機能 | Isolation total RNA from <150 mg fruit or seed using chloroform and Plantzol reagent |
| アプリケーション | RT-PCR, qPCR, Northern hybridization, Poly A purification, nucleic acid protection and in vitro translation translation |
| 精製方法 | Mini スピンカラム |
| 精製技術 | シリカ技術, DNA filtration technology |
| 加工方法 | マニュアル (遠心分離または真空) |
| サンプルの種類 | Simple plant, petal, peel, seed |
| サンプル量 | 50-150mg |
| 溶出量 | ≧300μl |
| 実行あたりの時間 | ≤15 minutes（1-24 samples） |
| カラムあたりの液体搬送量 | 800µl |
| カラムの結合収量 | 100μg |
原理
The Kit isolates total RNA from up to 150mg plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 15 分. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 300µl water using the Kit.
利点
- Efficient DNA removal – one step RNA抽出 can effectively remove genomic DNA
- High quality – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- 速い – several samples can be extracted in 15 minutes by column method
- Wide range – samples including animals, 植物, 細菌, 細胞, 等
- High output – large sample amount, and the yield can be up to 200μg
キット内容
| コンテンツ | R401402 | D401403 |
| 浄化時間 | 50 準備 | 250 準備 |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml 採取管 | 50 | 250 |
| PlantZol Reagent | 60 ミリリットル | 200 ミリリットル |
| Buffer GXP | 30 ミリリットル | 150 ミリリットル |
| Buffer RW1 | 50 ミリリットル | 200 ミリリットル |
| Buffer RW2* | 20 ミリリットル | 2 バツ 50 ミリリットル |
| RNase Free Water | 10 ミリリットル | 30 ミリリットル |
保管と安定性
The Kit can be stored dry at room temperature (15–25℃) 少なくとも一定期間は安定しています 18 この状況下で数か月. During shipment, crystals or precipitation may form in the Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.
レビュー
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