HiPure HP Plant DNA Maxi Kit

This product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 3g of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (ゲノム, ミトコンドリア, and chloroplast) for reliable PCR and southern blot in less than 1 時間.

詳細

仕様

原理

This product is based on silica Column purification. Plant material is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated. Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged.DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.

利点

• Broad spectrum – suitable for extracting DNA from various plant samples
• 速い – several samples can be extracted in 30 minutes by silica technology
• 高純度 – high quality DNA, completely remove inhibitors
• 高収量 – silica technology can achieve the highest yield

キット内容

保管と安定性

RNase A should be stored at 2-8°C upon arrival. しかし, 短期保管 (まで 12 週) 室温で (15-25℃) パフォーマンスには影響しません. 残りのキットコンポーネントは乾燥した状態で室温で保管できます。 (15-25℃) 少なくとも一定期間は安定しています 18 この状況下で数か月. キット全体は 2 ～ 8°C で保存できます。, ただし、この場合、バッファーは使用前に再溶解する必要があります。. 使用するときはすべてのバッファーが室温であることを確認してください.

購入ガイド