導入

Hipure Microbial RNA kit is suitable for extracting high-purity total RNA from bacterial/yeast culture medium. This kit combines high-efficiency Magzol Reagent (one-step extraction reagent) and silicagel column purification technology to complete the extraction of high-purity total RNA in only 40 分. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.

詳細

仕様

原理

Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of a high concentration of ionizing agent (such as グアニジン hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

利点

• 速い – several samples can be extracted in 30 分
• 高純度 – the purified RNA can be directly used in various downstream applications
• 高い回復力 – RNA can be recovered at the level of PG
• Good repeatability – silica gel column purification technology can obtain ideal results every time
• Broad spectrum – it can deal with various bacteria, including Gram-positive bacteria that are difficult to be lysed
• Sufficient components – the kit contains carried wall-breaking enzymes and glass beads

キット内容

保管と安定性

Buffer PHC should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. しかし, 短期保管 (DNase I up to 1 週, Buffer PHC up to 12 週) 室温で(15–25℃) 彼らのパフォーマンスには影響しない. The remaining kit components can be stored at roomtemperature (15–25℃) 少なくとも一定期間は安定しています 18 この状況下で数か月.