導入

This kit is used for extracting total pathongen nucleic acid from low cell content biological samples such as body fluid, 血清, プラズマ, immersion solution, tissue homogenate, culture, 等. The purified DNA/RNA is ready for downstream clinical in vitro detection such as Real Time PCR, biochip analysis, NGS and other related detection.

詳細

仕様

原理

This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA/RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. タンパク質やその他の不純物を洗浄した後, DNA / RNA is finally eluted with low-salt buffer (10 MmTris, pH 8.0).

利点

• 速い – column purification, several samples can be finished in 30 分
• High quality – high purity total RNA/DNA can be directly used in various sensitive downstream applications
• Safe – no phenol chloroform extraction required
• Sensitive – DNA/RNA can be recovered at the level of PG

キット内容

保管と安定性

Proteinase K and DNase I (粉) should be stored at 2–8°C upon arrival. しかし, 短期保管 (DNase I up to 1 week, Proteinase K up to 8 週) 室温で (15–25℃) does not affecttheir performance. The remaining kit components can be stored at room temperature (15–25℃) and are stable for 18 この状況下で数か月.