HiPure SF Plant DNA Kit

This product provides a fast and easy way to purify DNA from plant and fungal tissue. まで 100 mg of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (ゲノム,ミトコンドリア, and chloroplast) for reliable PCR and southern blotting in less than 1 時間. Purification requires no phenol or chloroform extraction oralcohol precipitation and involves minimal handling.

詳細

仕様

原理

Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated.Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.

利点

• Safe – require no phenol or chloroform extraction
• 速い – several samples can be extracted in 30 minutes by silica technology
• 高純度 – high quality DNA, completely remove inhibitors
• 高収量 – silica technology can achieve the highest yield

キット内容

保管と安定性

RNase A should be stored at 2-8°C upon arrival. しかし, 短期保管 (まで 24 週) 室温で (15-25℃) パフォーマンスには影響しません. 残りのキットコンポーネントは乾燥した状態で室温で保管できます。 (15-25℃) 少なくとも一定期間は安定しています 18 この状況下で数か月.

実験データ

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