HiPure Soil RNA Kit

導入

HiPure Soil RNA Kit is suitable for extracting high-purity microbial total RNA from soil samples. The kit adopts silica gel column purification technology and original humic acid adsorbent technology. It is suitable for extracting high-yield and high-purity total RNA from various soil samples, such as forest soil, grassland soil, mining soil, sediment and so on. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.

詳細

仕様

原理

The hipure silica gel column is based on a glass fiber filter membrane with a high binding force. Under the condition of a high concentration of ionizing agent (such as グアニジン hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bonds and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

利点

• High quality – high purity total RNA can be directly used in various sensitive downstream applications
• 速い – extraction of several samples can be completed in 60 minutes by the column purification method
• Safe – no phenol-chloroform extraction required
• Sensitive – RNA can be purified at the level of PG

キット内容

保管と安定性

Buffer PHC should be stored at 2–8°C upon arrival. しかし, 短期保管 (まで 12 週) 室温で (15–25℃) 彼らのパフォーマンスには影響しない. 残りのキットコンポーネントは乾燥した状態で室温で保管できます。 (15–25℃) 少なくとも一定期間は安定しています 18 この状況下で数か月. The entire kit can be stored at 2–8°C, しかしこの場合, バッファーは使用前に再溶解する必要があります. 使用するときはすべてのバッファーが室温であることを確認してください.

実験データ