HiPure Soil/Stool DNA 96 キット

• Isolation of High-Quality Genomic DNA: The product enables rapid and reliable isolation of high-quality genomic DNA from various soil and stool samples.
• Sample Processing Capacity: Capable of processing up to 100mg of stool samples and 500 mg of soil samples within 60 分.
• Innovative System Design: Combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of スピンカラム テクノロジー.
• Elimination of PCR Inhibitors: Effectively eliminates PCR-inhibiting compounds such as humic acid from soil samples during the purification process.
• Suitability for Various Applications: The purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing.
• Reduction in Organic Extractions: No organic extractions are required, thus reducing plastic waste and hands-on time.
• Increased Efficiency: Allows for the processing of multiple samples in parallel, enhancing overall workflow efficiency.

仕様

原理

• Sample Homogenization and Lysis: Soil or stool samples are homogenized and treated in a specially formulated buffer containing detergent. This process effectively lyses bacteria, yeast, and fungal samples.
• Removal of Contaminants: Our proprietary Absorber Solution is utilized to remove humic acid, proteins, polysaccharides, and other contaminants from the lysed sample.
• DNA Binding and Column Application: After adjusting binding conditions, the treated sample is applied to a DNA Mini Column, facilitating the binding of DNA to the column matrix.
• Rapid Wash Steps: Two rapid wash steps are employed to remove trace contaminants, ensuring the purity of the extracted DNA.
• Elution of Purified DNA: Pure DNA is eluted from the column in a low ionic strength buffer, providing high-quality, purified DNA ready for downstream applications.
• Direct Use in Downstream Applications: The purified DNA can be directly used in downstream applications without the need for further purification steps, streamlining laboratory workflows and saving time.

利点

• 速い – several samples can be extracted in 40 分 (after digestion)
• 高純度 – the purified DNA can be directly used in various downstream applications
• Good repeatability – silica technology can obtain ideal results every time
• 高い回復力 – DNA can be recovered at the level of PG

キット内容

保管と安定性

• Absorber Solution Storage: Upon arrival, the Absorber Solution should be stored at 2-8°C. しかし, it can also be stored at room temperature (15-25℃) for short-term periods (まで 24 週) without affecting its performance.
• Remaining Kit Components: The remaining components of the kit can be stored dry at room temperature (15-25℃). They remain stable for at least 18 この状況下で数か月, ensuring their efficacy and performance over an extended period.