ハイピュアティッシュ&Blood DNA Maxi カラムキット

• Challenges in DNA Extraction from Blood Samples:
  1. High Infectious Risk: Blood samples pose a significant infectious hazard to operators and the environment.
  2. Complex DNA Source: Blood DNA contains various types such as mitochondrial, ゲノム, and circulating DNA, leading to potential loss during extraction.
  3. Presence of Impurities: Blood samples contain impurities and inhibitory factors that can interfere with DNA extraction and downstream applications.
• Existing Extraction Methods:
  • Phenol-chloroform extraction, salting out, 等.
  • These methods typically involve pre-treatment steps to remove red blood cells and isolate white blood cells, which may result in loss of nucleic acid information.
• Product Benefits:
  • Fast and Easy Purification: Provides a rapid and straightforward method for total DNA purification.
  • Suitability: Purifies total DNA (ゲノム, バイラル, ミトコンドリア) from whole blood, 組織, そして培養細胞.
  • 応用: Ensures reliable DNA for PCR and Southern blotting assays.

仕様

原理

1. Cell Breakdown: Your sample is lysed (broken open) and digested with enzymes to release DNA into solution.
2. Capture: The mixture is passed through a silica column. DNA sticks to the silica membrane, while proteins and other impurities don’t.
3. Washing Away Impurities: The column is washed to remove any remaining proteins and other unwanted molecules.
4. DNA Release: Pure DNA is eluted (washed off) the silica membrane with a special buffer solution. This purified DNA is then ready for further analysis.

利点

• High-quality DNA – meets a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, 等.
• 速い – without separation of leukocytes, organic extraction, or ethanol precipitation
• 単純 – all nucleic acids can be obtained by direct digestion
• Pertinence – specially designed for isolating DNA from 3-10ml blood and related body fluids
• Wide applicability – handle a variety of liquid samples
• 経済 – excellent cost-effectiveness performance

キット内容

保管と安定性

Storage Recommendations:

• Proteinase K and RNase A: Upon arrival, 2～8℃で保存. 短期保管 (まで 12 週) 室温で (15-25℃) パフォーマンスに影響を与えることなく許容可能.
• Remaining Kit Components: Can be stored at room temperature (15-25℃) and remain stable for at least 18 この状況下で数か月.

購入ガイド