ハイピュアティッシュ&血液DNAマキシキット

製品概要

• Offers fast and easy purification methods for total DNA.
• Ensures reliable PCR and Southern Blotting results.
• Can purify various types of DNA: ゲノム, バイラル, and mitochondrial.
• Suitable for purification from whole blood, 組織, そして培養細胞.

仕様

原理

• Utilizes silica column purification method.
• The sample is lysed and digested with lysate and protease, releasing DNA into the lysate.
• Lysate is transferred to an adsorption column.
• Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed through filtration.
• Proteins and other impurities are washed away.
• Nucleic acid is finally eluted with low-salt buffer (10mmトリス, pH 9.0, 0.5mm EDTA).

利点

• High-quality DNA – meets a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, 等.
• 速い – without separation of leukocytes, organic extraction, or ethanol precipitation
• 単純 – all nucleic acids can be obtained by direct digestion
• Pertinence – specially designed for isolating DNA from 3-10ml blood and related body fluids
• Wide applicability – handle a variety of liquid samples
• 経済 – excellent cost-effectiveness performance

キット内容

保管と安定性

• Proteinase K and RNase A should be stored at 2-8°C upon arrival.
• Short-term storage at room temperature (15-25℃) for up to 12 weeks does not affect their performance.
• The remaining kit components can be stored at room temperature (15-25℃) and remain stable for at least 18 この状況下で数か月.

購入ガイド