Blood is a valuable source of DNA for clinical diagnostics and research, but traditional extraction methods present challenges:
- Safety Hazards: Bloodborne pathogens pose a risk of infection during extraction.
- DNA Loss: Red blood cell removal can eliminate desired DNA fractions (バイラル, circulating DNA).
- Impurities and Inhibitors: Blood components can interfere with downstream applications (PCR, Southern blotting).
This product offers a novel DNA extraction solution that addresses these issues:
- Safe and Efficient: No red blood cell removal minimizes infectious waste and contamination risks.
- Comprehensive DNA Recovery: Purifies total DNA (ゲノム, ミトコンドリア, バイラル, circulating) for a complete picture.
- Broad Sample Compatibility: Works with whole blood (fresh/frozen), プラズマ, 血清, バフィーコート, そして培養細胞.
- ダウンストリームアプリケーション: Purified DNA is suitable for PCR and Southern blotting.
This innovative method simplifies blood DNA extraction while ensuring safety, efficiency, and comprehensive DNA recovery. It represents a significant advancement for researchers in diagnostics, forensics, and medical research.
注記: This bulletin corrects the previous statement about sample compatibility. This method is not suitable for tissue samples.
仕様
| 特徴 | 仕様 |
| 主な機能 | Isolation of total DNA from 2ml blood and 200mg tissue using Midi column |
| アプリケーション | PCR, サザンボルトとウイルスの検出, 等 |
| 精製方法 | ミディ スピンカラム |
| 精製技術 | シリカ技術 |
| 加工方法 | マニュアル (遠心分離または真空) |
| サンプルの種類 | 組織, 細胞, 血, 唾液, swabs, blood spot, semen, and other clinical samples |
| サンプル量 | 0.2-2 ミリリットル |
| 溶出量 | ≧300μl |
| 実行あたりの時間 | ≤80 minutes |
| カラムあたりの液体搬送量 | 4ミリリットル |
| カラムの結合収量 | 1mg |
Principles
This product offers a streamlined method for purifying DNA from blood samples using silica column technology.
The Process:
- Lysis and Digestion: The blood sample is treated with a lysate solution and protease to break open cells and degrade proteins, releasing DNA into the solution.
- Binding to Silica: The lysate is transferred to a silica column. The silica membrane in the column selectively binds DNA molecules, while proteins and other impurities pass through.
- Washing: Wash buffers remove unbound proteins and cellular debris that didn’t adhere to the silica.
- Elution: A low-salt buffer (typically 10 mM トリス, pH 9.0, そして 0.5 mM EDTA) is used to elute the purified DNA from the silica. This disrupts the interaction between DNA and the silica, allowing the collection of purified DNA in a separate tube.
利点
- High-quality DNA – meets a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, 等.
- 速い – without separation of leukocytes, organic extraction, or ethanol precipitation
- Simple – all nucleic acids can be obtained by direct digestion
- Wide applicability – handle a variety of liquid samples
キット内容
| コンテンツ | D311302 | D311303 |
| 浄化時間 | 20 | 100 |
| HiPure gDNA Midi Columns | 20 | 100 |
| 15ml 採取管 | 40 | 200 |
| Buffer ATL | 50 ミリリットル | 250 ミリリットル |
| バッファAL | 50 ミリリットル | 250 ミリリットル |
| バッファGW1* | 22 ミリリットル | 110 ミリリットル |
| Buffer GW2* | 12 ミリリットル | 50 ミリリットル |
| RNase A | 20 mg | 90 mg |
| プロテイナーゼK | 100 mg | 440 mg |
| プロテアーゼ溶解バッファー | 10 ミリリットル | 30 ミリリットル |
| Buffer AE | 20 ミリリットル | 120 ミリリットル |
保管と安定性
For optimal performance:
- Store Proteinase K and RNase A at 2-8°C (refrigerator) upon arrival.
Acceptable short-term storage:
- Proteinase K and RNase A can be stored for up to 12 weeks at room temperature (15-25℃) without affecting their performance.
Remaining kit components:
- Store all other components of the kit dry at room temperature (15-25℃).
- They are stable for at least 18 この状況下で数か月.
重要な注意事項:
- If storing the entire kit at room temperature, ensure buffers are redissolved before use and always warm all buffers to room temperature before using them.
購入ガイド
| 名前 | カタログ番号 | サンプル量 | Leukocyte protocol* | Colum type | 溶出量 | Average yield | 実行あたりの時間 |
| HiPure Blood DNA ミニキット | D3111 | 10-200μl | 1ミリリットル | 2mlカラム | ≥20μl | 5-9μg/200μl | 30分以内 |
| ハイピュアティッシュ&Blood DNA Midi Kit | D3113 | 0.2-2ミリリットル | 10ミリリットル | 1.5mlカラム | ≧300μl | 20-40μg/1m | ≤80 minutes |
| ハイピュアティッシュ&Blood DNA Maxi Kit | D3115 | 3 -10ミリリットル | 10ミリリットル | 15mlカラム | ≥700μl | 20-40μg/1m | ≤90 minutes |
| ハイピュアティッシュ&血液DNA 96 キット | D3117 | 1-200μl | 1ミリリットル | 96 ウェルプレート | 3-8μg/200μl |
