This product offers a convenient and rapid method to purify total DNA from a wide range of samples, making it ideal for various downstream analyses:
- Fast and Efficient: The protocol is streamlined for quick DNA extraction with minimal hands-on time.
- High-Quality DNA: The system isolates total DNA (ゲノム, バイラル, ミトコンドリア) with high purity, suitable for reliable PCR, 定量的PCR (qPCR), Southern blotting, and viral DNA detection.
- Broad Sample Compatibility: The method effectively purifies DNA from a variety of sources, 含む:
- 組織
- 細胞
- 血
- Saliva
- Swabs
- Blood Spots
- Semen
- Other clinical samples
- ダウンストリームアプリケーション: The purified DNA can be used directly in various research techniques, 含む:
- PCR (ポリメラーゼ連鎖反応)
- qPCR (定量的PCR)
- Southern Blotting
- Viral DNA Detection
- Other DNA-based analyses
This solution simplifies DNA extraction while maintaining high quality, making it a valuable tool for researchers working in various fields of molecular biology, forensics, and diagnostics.
仕様
| 特徴 | 仕様 |
| 主な機能 | Isolation of total DNA from tissue/blood/body fluid/swab/dry blood spots |
| アプリケーション | PCR, qPCR, サザンボルトとウイルスの検出, 等 |
| 精製方法 | Mini スピンカラム |
| 精製技術 | シリカ技術 |
| 加工方法 | マニュアル (遠心分離または真空) |
| サンプルの種類 | 組織, 細胞, 血, 唾液, 綿棒, 血痕, semen, and other clinical samples |
| サンプル量 | Solid tissue: 1-10mg; 抗凝固剤の血液: 200μl |
| 溶出量 | ≥20μl |
| 実行あたりの時間 | 30 – 60 分 |
| カラムあたりの液体搬送量 | 800µl |
| カラムの結合収量 | 100μg |
原理
- Purification Method: Utilizes silica column purification for efficient nucleic acid extraction.
- Sample Lysis and Digestion: Samples are lysed and digested using lysate and protease, facilitating the release of DNA into the lysate.
- Nucleic Acid Adsorption: The lysed sample is transferred to an adsorption column, where nucleic acids selectively adsorb onto the membrane.
- Protein Removal: Proteins, which do not adsorb to the membrane, are removed through filtration, ensuring purity of the extracted nucleic acid.
- Washing Step: Proteins and other impurities are washed away from the membrane to enhance the purity of the extracted nucleic acid.
- Elution: Nucleic acid is finally eluted from the membrane using a low-salt buffer (10mmトリス, pH 9.0, 0.5mm EDTA), resulting in efficient recovery of high-quality nucleic acid.
利点
- High-quality DNA – meets a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, 等.
- 速い – without separation of leukocytes, organic extraction, or ethanol precipitation
- 単純 – all nucleic acids can be obtained by direct digestion
- Wide applicability- handle a variety of liquid samples
キット内容
| コンテンツ | D301802 | D301803 |
| 浄化時間 | 50 | 250 |
| HiPure DNA Mini Columns I | 50 | 250 |
| 2ml 採取管 | 100 | 500 |
| バッファATL | 30 ミリリットル | 150 ミリリットル |
| バッファAL | 30 ミリリットル | 150 ミリリットル |
| Buffer GW1 | 22 ミリリットル | 88 ミリリットル |
| Buffer GW2 | 12 ミリリットル | 50 ミリリットル |
| プロテイナーゼK | 24 mg | 120 mg |
| プロテアーゼ溶解バッファー | 1.8 ミリリットル | 10 ミリリットル |
| バッファAE | 15 ミリリットル | 60 ミリリットル |
保管と安定性
- Proteinase K Storage: Upon arrival, store Proteinase K at 2-8°C. 短期保管 (まで 12 週) 室温で (15-25℃) パフォーマンスに影響を与えることなく許容可能.
- Remaining Kit Components Storage: The remaining kit components can be stored at room temperature (15-25℃) and remain stable for at least 18 この状況下で数か月.
- Whole Kit Storage: The entire kit can also be stored at 2-8°C. しかし, in this case, バッファーは使用前に再溶解する必要があります. Ensure all buffers are at room temperature when used for optimal performance.
実験データ
