HiPure ユニバーサル DNA キット

製品概要

• Suitable for rapid extraction of total DNA from various sources: 組織, 細胞, 血, 唾液, 綿棒, 血痕, semen, and other clinical samples.
• Extracted DNA can be used directly for PCR, 定量的PCR, サザンブロット, virus DNA testing, and other applications.

仕様

原理

• Utilizes silica column purification method.
• The sample is lysed and digested with lysate and protease, releasing DNA into the lysate.
• Lysate is transferred to an adsorption column.
• Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and removed through filtration.
• Proteins and impurities are washed away.
• Nucleic acid is finally eluted with low-salt buffer (10mmトリス, pH 9.0, 0.5mm EDTA).

利点

• High-quality DNA – meets a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, 等.
• 速い – without separation of leukocytes, organic extraction, or ethanol precipitation
• 単純 – all nucleic acids can be obtained by direct digestion
• Wide applicability- handle a variety of liquid samples

キット内容

保管と安定性

• Proteinase K should initially be stored at 2-8°C upon arrival.
• Short-term storage at room temperature (15-25℃) for up to 12 weeks does not affect its performance.
• The remaining kit components can be stored at room temperature (15-25℃) and remain stable for at least 18 月.
• Alternatively, the entire kit can be stored at 2-8°C, but buffers should be redissolved before use.
• Ensure all buffers are at room temperature when used.

実験データ