導入
This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, 血清, プラズマ, urine, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.
詳細
仕様
| 特徴 | 仕様 |
| 主な機能 | Extract viral RNA/DNA from non-cell/low cell content biological samples |
| アプリケーション | RT-PCR，PCR，NGS |
| 製品 | Viral total nucleic acid |
| 精製方法 | Mini スピンカラム |
| 精製技術 | シリカ技術 |
| 加工方法 | マニュアル (遠心分離または真空) |
| サンプルの種類 | Cell-free body fluid such as plasma, 血清, soaking solution and tissue homogenate supernatant |
| サンプル量 | 200μl |
| 収率 | 2-10μg |
| 溶出量 | ≥30μl |
| 実行あたりの時間 | 30分以内 |
| カラムあたりの液体搬送量 | 800μl |
| カラムの結合収量 | 100μg |
原理
This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA / RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. タンパク質やその他の不純物を洗浄した後, DNA / RNA was finally eluted with low-salt buffer (10 Mm Tris, pH 8.0).
利点
- 速い – several samples can be extracted in 20 minutes by column method
- High quality – high purity total RNA can be directly used in various sensitive downstream applications
- Safe – no phenol chloroform extraction required
- Sensitive – DNA/RNA can be recovered at the level of PG
- 高収量 – carrier RNA contained in the product maximize the recovery of trace nucleic acid
キット内容
| コンテンツ | IVD4173 |
| 浄化時間 | 100 準備 |
| HiPure バイラル ミニカラム | 100 |
| 2ml 採取管 | 200 |
| PK/Carrier RNA | 50 mg |
| プロテアーゼ溶解バッファー | 5 ミリリットル |
| バッファAL | 30 ミリリットル |
| Buffer MW1* | 44 ミリリットル |
| バッファーMW2* | 50 ミリリットル |
| RNase Free Water | 15 ミリリットル |
保管と安定性
Proteinase K should be stored at 2–8°C upon arrival. しかし, 短期保管 (Proteinase K up to 8 週) 室温で (15–25℃) 彼らのパフォーマンスには影響しない. The remaining kit components can be stored at room temperature (15–25℃) and are stable for 18 この状況下で数か月.
実験データ
レビュー
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