HiPure 酵母 DNA キット

This product provides a reliable solution for DNA isolation from yeast samples. Total DNA can be purified from yeast (<5バツ 10⁷) without phenol or chloroform. The whole extraction can be finished within 60 分. Purified DNA can be directly used for PCR, Southern blot, ect.

詳細

仕様

原理

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA がライセート中に放出される. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. タンパク質やその他の不純物を洗浄した後, Nucleic acid was finally eluted with low-salt buffer (10mmトリス,pH9.0, 0.5mm EDTA).

利点

• 速い – several samples can be extracted in 40 分 (after digestion)
• 高純度 – purified DNA can be directly used in various downstream applications
• Good repeatability – silica technology can obtain ideal results every time
• 高い回復力 – DNA can be recovered at the level of PG
• Sufficient components – lysozyme, protease K, RNase A and glass beads

キット内容

保管と安定性

Proteinase K should be stored at 2–8°C upon arrival. しかし, 短期保管 (まで 12 週) 室温で (15–25℃) 彼らのパフォーマンスには影響しない. 残りのキットコンポーネントは乾燥した状態で室温で保管できます。 (15–25℃) 少なくとも一定期間は安定しています 18 この状況下で数か月. Buffer ATL may precipitate at low temperature. Dissolve it by 37℃ water bath.