製品導入
This product offers a rapid and efficient method to isolate DNA from a wide range of samples, 含む:
- ティッシュ
- 細胞
- 血
- Saliva
- Swabs
- Blood Spots
- Semen
- And more!
The extracted DNA is ready for immediate use in downstream applications like:
- PCR (ポリメラーゼ連鎖反応)
- qPCR (定量的PCR)
- Southern Blotting
- Viral DNA detection
- Other DNA-based analyses
仕様
| 特徴 | 仕様 |
| 主な機能 | Isolation of total DNA from tissue/blood /body fluid /swab /dry blood spots |
| アプリケーション | PCR,qPCR, サザンボルトとウイルスの検出, 等. |
| 精製方法 | Mini スピンカラム |
| 精製技術 | シリカ技術 |
| 加工方法 | マニュアル (遠心分離または真空) |
| サンプルの種類 | 組織, 細胞, 血, 唾液, 綿棒, 血痕, semen, and other clinical samples |
| サンプル量 | Solid tissue:1-10mg, 抗凝固剤の血液:200メートル |
| 収率 | 1 -15μg |
| 溶出量 | ≥20μl |
| 実行あたりの時間 | 30 -60 分 |
| カラムあたりの液体搬送量 | 750μl |
| カラムの結合収量 | 100μg |
原理
- Lysis and Digestion: Begin by lysing and digesting the sample using a specialized lysate and protease combination. This step facilitates the release of DNA into the lysate, preparing it for the purification process.
- Adsorption Column: Transfer the lysate mixture to an adsorption column. Here, the nucleic acid selectively adheres to the silica membrane, while proteins and other contaminants remain unadsorbed. This selective binding ensures the efficient removal of impurities.
- Filtration: Employ filtration to efficiently remove the unadsorbed proteins and other contaminants from the mixture. This step further enhances the purity of the nucleic acid sample.
- Thorough Washing: Perform a thorough washing step to eliminate any remaining proteins and impurities, ensuring the purity of the nucleic acid sample.
- Elution: Finally, elute the purified nucleic acid from the adsorption column using a low-salt buffer solution (10mmトリス, pH 9.0, 0.5mm EDTA). This gentle elution process preserves the integrity of the DNA, readying it for downstream applications.
利点
- 多用途性: Suitable for a diverse range of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, などなど. Whether you’re performing basic research or advanced molecular assays, our DNA meets your requirements.
- Efficiency: Accelerate your workflow with fast DNA extraction. Say goodbye to time-consuming processes like leukocyte separation, organic extraction, or ethanol precipitation. Our method streamlines the extraction process for swift results.
- Simplicity: Enjoy a straightforward extraction process. No complex steps or specialized equipment are required. Obtain all nucleic acids directly through simple digestion, saving you time and effort in the lab.
- Broad Applicability: Our DNA extraction method is versatile and adaptable. It can handle various sample types, including liquid samples, animal tissues, そして培養細胞. Whether you’re working with blood, 組織, or cell cultures, our method delivers consistent results across different sample types.
キット内容
| コンテンツ | VD3018 |
| 浄化時間 | 100 |
| 2ml 採取管 | 2×100 |
| バッファATL | 60 ミリリットル |
| バッファAL | 60 m |
| Buffer GW1 | 44 m |
| Buffer GW2 | 50 m |
| プロテイナーゼK | 60 mg |
| プロテアーゼ溶解バッファー | 5 ミリリットル |
| Buffer AF | 15 m |
保管と安定性
- Proteinase K Storage:
- Upon arrival, 2～8℃で保存.
- 短期保管 (まで 12 週) 室温で (15-25℃) パフォーマンスに影響を与えることなく許容可能.
- Remaining Kit Components:
- Store at room temperature (15-25℃).
- Stable for at least 18 この状況下で数か月.
- Entire Kit Storage:
- Can be stored at 2-8°C.
- Ensure buffers are redissolved before use if stored at this temperature.
- Buffers should be at room temperature when used.
実験データ
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