MagPure A3 Features:
- Technology Base:
- Utilizes Magen’s solid-phase paramagnetic bead technology for the purification of PCR amplicons.
- High-Throughput Compatibility:
- Designed for high-throughput applications, facilitating efficient processing of multiple samples.
AmPure Technology:
- 選択的結合:
- Features an optimized buffer that selectively binds PCR amplicons of 100bp and larger to paramagnetic beads.
- 汚染物質の除去:
- Efficiently removes excess primers, ヌクレオチド, 塩, and enzymes through a simple washing procedure.
- Purity of PCR Product:
- Delivers PCR products that are essentially free of contaminants, enhancing downstream application reliability.
詳細
仕様
特徴 | 仕様 |
主な機能 | Selectively recover DNA from PCR products and enzymatic reaction solution (Replace Beckmen or agencourt AmPure) |
アプリケーション | Sequencing, gene chip and qPCR, 等. |
精製技術 | 磁気ビーズ技術 |
加工方法 | マニュアル (遠心分離または真空) |
サンプルの種類 | PCR products, enzymatic reaction solution |
サンプル量 | 適切な |
溶出量 | ≥10μl |
稼働時間 | 50分以内 |
原理
Product Purification Method:
- High Binding Magnetic Particle Method:
- Utilizes high binding magnetic particles for purification.
- PCR Amplicon Binding:
- PCR amplicons are mixed with MagPure A3.
- DNA fragments of 100bp and larger bind to magnetic beads.
- 汚染物質の除去:
- Excess primers, ヌクレオチド, 塩, and enzymes are removed via a simple washing procedure.
- DNA Elution:
- Purified DNA is eluted using Elution Buffer or Water.
利点
- Wide application – DNA製品, enzyme digestion system, PCR reaction
- 高い回復力 – suitable for 100bp-20kb fragments
- 高純度 – effectively remove dNTP, primer, primer dimer, salt ion and other impurities
- Strong applicability – manual operation or automatic workstation
キット内容
コンテンツ | BXP-5 | BXP-50 | BXP-500 |
MagPure A3 | 5 ミリリットル | 50 ミリリットル | 500 ミリリットル |
保管と安定性
Storage and Handling Instructions:
- Storage Conditions:
- Store MagPure A3 at 2-8°C upon arrival.
- Stable for up to 18 この状況下で数か月.
- Short-term Storage:
- 短期保管 (まで 12 週) 室温で (15-25℃) does not affect performance.
- Reagent Preparation:
- Shake the reagent well before use to ensure homogeneity.
- The reagent should appear homogenous and consistent.
凍らせないでください.
レビュー
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