MagPure A4 XP Features:
- Technology Base:
- Employs Magen’s solid-phase paramagnetic bead technology designed for the purification of PCR amplicons.
- High-Throughput Capability:
- Suitable for high-throughput settings, allowing for efficient simultaneous processing of multiple samples.
AmPure Technology:
- 選択的結合:
- Uses an optimized buffer that selectively binds PCR amplicons 100bp and larger to paramagnetic beads.
- 汚染物質の除去:
- Simple washing procedure efficiently removes excess primers, ヌクレオチド, 塩, and enzymes.
- Purity of PCR Product:
- Results in PCR products that are virtually free from contaminants, suitable for precise downstream applications.
詳細
仕様
特徴 | 仕様 |
主な機能 | Selectively recover DNA from PCR products and enzymatic reaction solution (Replace Beckmen or agencourt AmPure XP) |
アプリケーション | NGS, DNA library |
精製技術 | 磁気ビーズ技術 |
加工方法 | マニュアル (遠心分離または真空) |
サンプルの種類 | DNA製品, 制限エンドヌクレアーゼシステム, or other enzymatic reaction solutions |
サンプル量 | 適切な |
回復 | 80% |
稼働時間 | 50分以内 |
原理
Purification Method:
- High Binding Magnetic Particle Method:
- Utilizes high binding magnetic particles for purification.
- PCR Amplicon Binding:
- PCR amplicons are mixed with MagPure A3.
- DNA fragments of 100bp and larger bind to magnetic beads.
- 汚染物質の除去:
- Excess primers, ヌクレオチド, 塩, and enzymes are removed through a simple washing procedure.
- DNA Elution:
- Purified DNA is eluted using Elution Buffer or Water.
利点
- High recovery — up to 80%
- High throughput — using magnetic beads purification technology
キット内容
コンテンツ | BP-5 | BP-50 | BP-500 |
MagPure A4 XP | 5 ミリリットル | 50 ミリリットル | 500 ミリリットル |
保管と安定性
Storage and Handling Instructions:
- Storage Conditions:
- Store MagPure A4 XP at 2-8°C upon arrival.
- Stable for up to 18 この状況下で数か月.
- Short-term Storage:
- 短期保管 (まで 4 週) 室温で (15-25℃) does not affect performance.
- Reagent Preparation:
- Shake the reagent well before use to ensure homogeneity.
- The reagent should appear homogeneous and consistent in color.
凍らせないでください.
レビュー
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