MagPure Blood RNA Kit

This product supplies a simple and rapid extraction of total RNA from Blood, バフィーコート, 骨髄, cell suspension, and other body fluids. The kit is based on superparamagnetic particle purification technology, with no phenol-chloroform extraction or alcohol precipitation. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing, and so on.

詳細

仕様

原理

The Kit can be used for both manual extraction processes and automatic nucleic acid extraction machines. This Kit is suitable for extracting RNA from ≤5×10⁶ cells suspension, 200-300µl whole blood, 200µl buffy coat, 100µl bone marrow. この製品は高結合磁性粒子の精製方法に基づいています。. サンプルはライセートとプロテアーゼの作用により溶解および消化されます。. RNA/DNA is released into the lysate. 磁性粒子と結合溶液を添加した後, DNA/RNA will be adsorbed on the surface of magnetic particles, タンパク質などの不純物を吸着せずに除去します。. The adsorbed particles were digested using DNase and washed with washing buffer to remove proteins and impurities, エタノールで洗浄して塩を除去, そして最後に, RNA was eluted by Elution Buffer.

利点

• 高純度 – から 260 / 280:1.9-2.0, から 260 / 230:1.5-2.0
• 経済 – 未満 50% Qiagen およびその他の輸入製品の価格
• 自動 – extraction without manual participation, saving time and effort
• 高収量 – nucleic acid recovery up to 95%
• 一般的な – samples include whole blood, 血清, プラズマ, lymphocyte suspension, 骨髄, cultured cells, 等.

キット内容

保管と安定性

MagPure RNA Particles and Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. しかし, 短期保管 (DNase I up to 1 week, MagPure RNA Particles and Proteinase K up to 8 週) 室温で (15–25℃) 彼らのパフォーマンスには影響しない. The remaining kit components can be stored at room temperature (15–25℃) 少なくとも一定期間は安定しています 18 この状況下で数か月.