MagPure 循環 DNA Maxi キット

導入

This kit empowers researchers with a streamlined solution for isolating high-quality circulating cell-free DNA (cfDNA) from various biofluids:

• Sample Types: プラズマ, 血清 (1-6ミリリットル)
• Target: cfDNA molecules
• ダウンストリームアプリケーション:
  • PCR (polymerase chain reaction)
  • Real-time PCR
  • Biochip analysis
  • Next-generation sequencing (NGS)

仕様

原理

This product utilizes innovative magnetic bead technology for efficient DNA extraction:

1. Lysis and Digestion: The sample undergoes treatment with a special solution (lysate) and an enzyme (protease) to break open cells and degrade proteins, releasing DNA into the lysate.
2. Binding to Magnetic Beads: Magnetic particles coated for high DNA affinity are added to the lysate. DNA molecules selectively bind to the surface of these beads, while impurities like proteins remain unbound.
3. Washing and Purification: A series of washes with different solutions remove unbound proteins and other cellular debris.
4. Elution: A special elution buffer disrupts the interaction between the DNA and the magnetic beads, allowing the purified DNA to be collected in a separate tube.

利点

• 経済 – 未満 50% Qiagen およびその他の輸入製品の価格
• 自動 – without labor
• 高純度 – から 260 / 280: 1.7-1.9, から 260 / 230:1.5-2.0

キット内容

保管と安定性

Ideal Storage:

• Store Proteinase K, キャリアRNA, and MagPure Particles F in the refrigerator (2-8℃) upon arrival for maximum effectiveness.

Acceptable Short-Term Storage:

• These components can be kept at room temperature (15-25℃) for up to 12 weeks without impacting their performance.

Storing Remaining Components:

• All other kit components can be stored dry at room temperature (15-25℃).
• They will remain stable for at least 18 この状況下で数か月.

Important Notes:

• If storing the entire kit at 2-8°C, remember to redissolve buffers before use.
• Always ensure all buffers reach room temperature before using them for optimal results.

実験データ