導入

This kit is suitable for high-throughput extraction of total RNA from paraffin embedded tissue samples, miRNAを含む. The kit is based on superparamagnetic magnetic particle purification technology. The extraction process does not require the use of toxic phenolic chloroform extraction or time-consuming alcohol precipitation.The whole extraction process only takes 120 分. The obtained RNA can be directly used in RT-PCR. The high-throughput extraction can be realized by 96 hole magnetic frame and 96 holeo scillator, various nucleic acid extractors (such as KingFisherFle, MagMix32, マグミックス 96) or cooperating with the pipette workstation (Hamilton, Tican) for automatic extraction.

詳細

仕様

原理

この製品は高結合磁性粒子の精製方法に基づいています。. サンプルはライセートとプロテアーゼの作用により溶解および消化されます。. DNA がライセート中に放出される. 磁性粒子と結合溶液を添加した後, DNAは磁性粒子の表面に吸着されます, タンパク質などの不純物を吸着せずに除去します。. 吸着粒子を洗浄液で洗浄し、タンパク質や不純物を除去しました。, エタノールで洗浄して塩を除去, and finally DNA was eluted by Elution Buffer.

利点

• 速い – several samples can be extracted in 120 minutes by column method
• Safe – no phenol chloroform extraction required

キット内容

保管と安定性

Proteinase K and MagBind Particles should be stored at 2–8°C upon arrival. DNase I should bestored at -20°C. しかし, 短期保管 (DNase I up to 1 週, Proteinase K and MagBind Particles up to 8 週) 室温で (15–25℃) 彼らのパフォーマンスには影響しない. The remaining kit components can be stored at room temperature (15–25℃) 少なくとも一定期間は安定しています 18 months under theseconditions.