導入

This product is suitable for extracting total pathogen RNA from biological samples with no/low cell content such as body fluid, 血清, プラズマ, soaking solution, tissue homogenate supernatant, culture medium supernatant, 等. The purified RNA can be used for clinical in vitro detection.

It is special designed for Pathogen RNA抽出 from Sputum samples, can be used in tuberculosis (TB) detection.

詳細

仕様

原理

この製品は高結合磁性粒子の精製方法に基づいています。. サンプルはライセートとプロテアーゼの作用により溶解および消化されます。. 磁性粒子と結合溶液を添加した後, DNA/RNA will be adsorbed on the surface of magnetic particles, タンパク質などの不純物を吸着せずに除去します。. 吸着粒子を洗浄液で洗浄し、タンパク質や不純物を除去しました。, エタノールで洗浄して塩を除去, and finally DNA/RNA was eluted by Buffer NFW.

利点

• 速い – several samples can be extracted in 40 minutes by column method
• High quality – high purity total RNA / DNA can be directly used in various sensitive downstream applications
• Safe – no phenol chloroform extraction required
• Sensitive – DNA/RNA can be recovered at the level of PG

キット内容

保管と安定性

マグピュア粒子 and Proteinase K, DNase I should be stored at 2–8°C upon arrival. しかし, 短期保管 (まで 2 週) 室温で (15–25℃) 彼らのパフォーマンスには影響しない. The remaining kit components can be stored at room temperature (15–25℃) and are stable for 18 この状況下で数か月.