導入

The kit offers the unique feature to isolate total RNA including small RNA and DNA from serum and plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time consuming proteinase digest. RNA purified using the kit is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

詳細

仕様

原理

この製品は高結合磁性粒子の精製方法に基づいています。. The sample material is denatured in Lysis Buffer. The protein is then precipitated by Protein Precipitation Solution and pelleted by centrifugation. 磁性粒子と結合溶液を添加した後, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption.The adsorbed particles were washed with washing solution to remove proteins and impurities, エタノールで洗浄して塩を除去, and finally RNA was eluted by Elution Buffer.

利点

• Safe – no phenol chloroform extraction and no use of Trizol reagent
• 速い – several samples can be extracted in 60 minutes by column method

キット内容

保管と安定性

MagPure Particle N should be stored at 2–8°C upon arrival. しかし, 短期保管 (まで 8 週) 室温で (15–25℃) 彼らのパフォーマンスには影響しない. The remaining kit components can be stored at room temperature (15–25℃) 少なくとも一定期間は安定しています 18 この状況下で数か月.