MagZol Reagent is a reagent system for the isolation of total RNA from cells and tissues. The reagent, a single-phase solution consisting of phenol and グアニジン isothiocyanate, is modification of the single-step RNA isolation method developed by Chomczynski and Sacchi. The sample is homogenized and lysed in MagZol Reagent which maintains the integrity of the RNA, while disrupting and denaturing endogenous RNases and other cellular components. Extraction of the lysate with chloroform further denatures proteins and separates the mixture into an organic and an aqueous phase. RNA remains exclusively in the aqueous phase, and is subsequently recovered by isopropanol.
This method is suitable for small quantities of tissue (<100mg) and cells (<5 X106), and large quantities of tissue (up to 1g) and cells (<108), of human, animal, 植物, or bacterial origin. The simplicity of the MagZol Reagent method allows simultaneous processing of a large number of samples. The entire procedure can be completed in one hour. Total RNA prepared in this manner can be used for Northern blot analysis, dot blot hybridization, ポリ(あ) + selection, in vitro translation, RNase protection assay, and molecular cloning. For use in amplification by thermal cycling, treatment of the isolated RNA with RNase-free DNase I is recommended when the two amplimers lie within a single exon.
詳細
仕様
| 特徴 | 仕様 |
| 主な機能 | Extract RNA from liquid samples by salting out method |
| アプリケーション | RT-PCR, Northern hybridization, ポリ (ある) enrichment, 等. |
| 精製技術 | Acid phenol guanidine isothiocyanate |
| 加工方法 | マニュアル (遠心分離) |
| サンプルの種類 | Various liquid samples |
| サンプル量 | Flexible |
| 溶出量 | Variation with sample size |
| 実行あたりの時間 | Variation with sample size |
利点
- Flexible – sample amount can be adjusted according to the demand
- Cost performance -the most economical nucleic acid extraction technology
保管と安定性
MagZol Reagent should be stored at 2-8°C upon arrival and is stable for at least 24 条件下で数か月. しかし, 短期保管 (まで 12 週) 室温で (15-25℃) パフォーマンスには影響しません.
レビュー
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