Sanshibio Taq Hot-Start DNA Polymerase

Taq Hot-Start DNA Polymerase

商品番号：EH001 仕様：500U/1000U/5000U Storage: -20℃で保存

製品導入：

This product is an antibody-blocked hot-start enzyme that suppresses non-specific amplification caused by primer misannealing or primer dimer formation at low temperatures. It is suitable for Hot Start PCR. Since the antibody is inactivated during the initial DNA denaturation step of the PCR reaction, there is no need for special deactivation treatment; it can be used under standard PCR conditions. The PCR product amplified using this product has an ‘A’ base added to the 3′ end, making it directly clonable into a T-Vector. It can also be cloned into blunt-end vectors after end polishing and phosphorylation.

商品内容：

ストレージ:

-20℃で保存, with a shelf life of at least 12 月.

アクティビティの定義:

活性化されたマヒマヒ精子 DNA をテンプレート/プライマーとして使用する, アクティビティは次のように定義されます 1 ユニット (U) 酸不溶性物質が組み込まれている, 取り上げることで 10 ヌクレオチドの nmol 30 74℃で5分.

品質管理:

この製品は品質検査を受けており、デオキシリボヌクレアーゼ・エンドヌクレアーゼ活性を含んでいません。, デオキシリボヌクレアーゼ エキソヌクレアーゼ活性, リボヌクレアーゼの汚染. 宿主ゲノムDNA残存量は以下の通り 10 コピー.

製品の用途:

Hot Start PCR; DNA sequence determination.

使用説明書:

• Allow the required reagents to equilibrate at room temperature until fully dissolved. Gently mix well (ボルテックスしないでください), and use after brief centrifugation to prevent excessive bubble formation and avoid repeated freeze-thaw cycles. If frequently used, 4℃で保存. Prepare the PCR reaction mixture according to the following components (prepare the reaction mixture on an ice box):

Recommended Reaction System:

注記: The amounts of each component in the reaction system can be adjusted according to actual requirements.

• 一般的に, 反応には二段階法を使用できます; if the two-step amplification is not satisfactory, a three-step method can be used to set up the PCR reaction program.

注記: Reaction conditions can be adjusted and optimized according to actual requirements.

• 反応が完了した後, analyze the experimental results. For detailed analysis methods, refer to the PCR amplification instrument operating manual.

予防:

1. Please choose an appropriate annealing (拡大) temperature based on the primer design. 通常, the Tm value of the primers is designed to be around 60°C. For primers with lower annealing temperatures or for amplifying long fragments exceeding 200 血圧, 3 段階の方法が推奨されます. The extension time can be adjusted based on the length of the PCR product, GC content, and other factors. The extension time per kb of product is closely related to template complexity. Simple templates use 20 秒, normal templates use 30 秒, and complex templates use 1 分.
2. The PCR reaction conditions should be set differently based on factors such as template, primers, length of the PCR product, and GC content. The final concentration of commonly used primers can be adjusted within the range of 0.2-1.0 μM. The DNA template concentration can also be adjusted based on its concentration. For complex templates or high GC content, consider extending the denaturation/annealing or extension times, and increasing denaturation/annealing temperature.
3. Use dedicated areas and pipettors before and after amplification, 手袋を着用する, そして頻繁に変更する. After PCR amplification, do not open the reaction tubes directly. Place them at 4°C or -20°C to cool sufficiently before opening to minimize the risk of PCR product contamination in the experimental environment.