- 製品説明:
- Designed for the purification of high-molecular-weight genomic or mitochondrial DNA.
- Suitable for various blood samples.
- Sample Types:
- Whole blood
- Buffy coat
- Bone marrow
- Body fluids
- Purification Time: As little as 25 分.
- 特徴:
- Convenient and scalable purification procedure.
- Removes contaminants and enzyme inhibitors like proteins and divalent cations.
- Purified DNA is ready for immediate use in sensitive downstream applications or for archiving.
仕様
| 特徴 | 仕様 |
| 主な機能 | Isolation of total DNA from whole blood using the economic salt out method |
| アプリケーション | PCR, enzyme digestion, Southern hybridization, 等. |
| 精製技術 | Salting out method |
| 加工方法 | マニュアル (遠心分離または真空) |
| サンプルの種類 | Animal tissue |
| サンプル量 | Unlimited |
| 溶出量 | ≥100μl |
| 実行あたりの時間 | Variation with sample amount |
Principles
- Lysis Process:
- Cells are lysed with an anionic detergent in the presence of a DNA stabilizer.
- The DNA stabilizer limits the activity of intracellular DNases and DNases in the environment.
- RNA Removal:
- RNA is eliminated by treatment with an RNA-digesting enzyme.
- 汚染物質の除去:
- Other contaminants, like proteins, are removed by salt precipitation.
- DNA Recovery:
- Genomic DNA is recovered by precipitation with alcohol.
- The recovered DNA is dissolved in Buffer TE.
- Quality Assurance:
- Purified DNA typically exhibits an A260/A280 ratio between 1.7 そして 1.9.
- DNA size can reach up to 200 kb.
利点
- Safe – without phenol-chloroform extraction
- Environment friendly – the reagents used are safe, non-toxic, and without pollution
- High molecular weight – the molecular weight of genomic DNA is about 50-150kb
- 高純度 – the purified DNA has A260/280=1.8-1.9, A260/230=2.0-2.5
- Unlimited sample size – solution type operation, sample volume can be adjusted at will
- Cost performance – the most economical nucleic acid extraction technology
キット内容
| コンテンツ | D331101 | D331102 | D331103 |
| Purification Volumes | 50 ミリリットル | 300 ミリリットル | 1000 ミリリットル |
| 10 x Buffer RBC | 20 ミリリットル | 100 ミリリットル | 3 バツ 100 ミリリットル |
| Buffer WTL | 60 ミリリットル | 350 ミリリットル | 1000 ミリリットル |
| Buffer PPS | 20 ミリリットル | 120 ミリリットル | 350 ミリリットル |
| RNase ソリューション | 300 µl | 1.8 ミリリットル | 6 ミリリットル |
| Buffer TE | 10 ミリリットル | 30 ミリリットル | 120 ミリリットル |
保管と安定性
- RNase Solution Storage:
- Upon arrival, 2～8℃で保存.
- Short-term storage (まで 24 週) 室温で (15-25℃) does not affect performance.
- Remaining Kit Components:
- Dry components can be stored at room temperature (15-25℃).
- Stable for at least 18 この状況下で数か月.
- Entire Kit Storage:
- キット全体は 2 ～ 8°C で保存できます。.
- Buffers should be redissolved before use if stored at this temperature.
- Ensure all buffers are at room temperature when used.
