導入
The products are the core raw material for nucleic acid extraction after strict screening and quality control.
Buffer solution is a kind of solution that can resist the influence of a small amount of strong acids and bases and maintain the pH value of the system unchanged.
It is very important to maintain pH 4.0-p11.0 in the process of nucleic acid extraction. Nucleic acid is easy to hydrolyze in an acidic solution and stable in a neutral or weak alkaline solution.
Tris, namely trimethylolaminomethane, pH buffer range: 7.0-9.0, can maintain the stability of the nucleic acid released after the cleavage of the sample to be extracted, to avoid the degradation of nucleic acid and improve the concentration and purity of nucleic acid.
Since the Tris reagent cannot be treated with DEPC, it must be strictly determined that there is no RNase in the preparation process of Tris containing reagent. Generally, it must be prepared with DEPC-treated water. したがって, Tris must be of high purity and free of DNase / RNase. This product is a high-purity product, more than 99.99%, and has been strictly tested by RNase / DNase quality control. There is no DNase activity and RNase activity.
詳細
仕様
特徴 | 仕様 | |
基本的な内容 | 推奨アプリケーション | 核酸抽出 |
CAS番号 | 77-86-1 | |
式 | C4H11NO3 | |
分子量 | 121.14 | |
Content | >99.5% | |
レベル | 分子生物学 | |
外観 | White crystalline particles | |
保存条件 | 室温 | |
安定性 | ドライコンディションでは無制限 | |
不純物パラメータ | 匂い | 何もない |
Solubility | 2mol/L colorless, clear and free of impurities | |
Heavy metals | ≤5 PPM | |
水分 | ≤0.2% | |
Sulfate | ≤10 PPM | |
Chloride | ≤3 PPM | |
鉄 | ≤1 PPM | |
紫外線吸収値 | 吸光度@ 230 (1M) | ≤6 |
吸光度@ 260 (1M) | ≤1 | |
吸光度@ 280 (1M) | ≤0.01 | |
Light absorption value @ 320 (1m) | ≤0.01 | |
核酸抽出関連 | DNase detection (0.5M) | 検出されず |
RNase detection (0.5M) | 検出されず | |
PH(2M) | 10.0-11.0 |
注文情報
カタログ番号. | 商品名 | パッケージ |
C11222 | Tris Base, Trizma base (分子生物学) | 1KG |
C11223 | 5KG |
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