導入
- The products undergo rigorous screening and quality control to serve as the primary raw material for nucleic acid extraction.
- Buffer solutions are capable of resisting the impact of strong acids and bases, thereby maintaining a stable pH within the system. It’s crucial to uphold a pH range of 4.0 に 11.0 during nucleic acid extraction.
- Nucleic acids are prone to hydrolysis in acidic conditions but remain stable in neutral or weakly alkaline environments. Tris, or trimethylolaminomethane, functions as a pH buffer within the range of 7.0 に 9.0. Its role is to preserve the stability of nucleic acids post-sample cleavage, preventing degradation and enhancing concentration and purity.
- Tris-containing reagents cannot undergo treatment with DEPC, necessitating rigorous assurance of RNase absence during Tris preparation. 通常, DEPC-treated water is employed for preparation to ensure RNase-free Tris of high purity, exceeding 99.99%.
- This product undergoes stringent testing for RNase and DNase activity, confirming its purity of over 99.99% and absence of DNase or RNase activity.
詳細
仕様
特徴 | 仕様 | |
基本的な内容 | 推奨アプリケーション | 核酸抽出 |
CAS番号 | 1185-53-1 | |
式 | C4H11NO3.HCL | |
分子量 | 157.60 | |
安定性 | ドライコンディションでは無制限 | |
レベル | 分子生物学 | |
外観 | ホワイトクリスタル | |
純度 | ≥99% | |
輸送条件 | 室温 | |
保存条件 | 室温 | |
不純物パラメータ | 匂い | 何もない |
Melting point ℃ | 145-155 | |
Heavymetal | <5 PPM | |
Losson drying | ≤0.09% | |
紫外線吸収値 | 吸光度@ 230 (5%) | ≤0.01 |
吸光度@ 260 (5%) | ≤0.01 | |
吸光度@ 280 (5%) | ≤0.01 | |
吸光度@ 320 (5%) | ≤0.01 | |
核酸抽出関連 | DNase テスト (0.5M) | 検出されず |
RNase検査 (0.5M) | 検出されず | |
PH value (2M) | 4.0-5.0 |
注文情報
カタログ番号. | 商品名 | パッケージ |
C11224 | Tris-HCl, TRIS hydrochloride (分子生物学) | 1KG |
C11225 | 5KG |
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