소개
- Guanidine hydrochloride is a crucial component in nucleic acid extraction, selected after stringent screening and quality control.
- It acts as a powerful denaturant and ionizing agent, enhancing the solubility of hydrophobic molecules and denaturing proteins.
- Guanidine hydrochloride is effective in denaturing and inactivating various enzymes and proteins.
- 안에 6 M guanidine hydrochloride solution, 그만큼 “secondary structure” of proteins, maintained by intermolecular non-covalent interactions, is disrupted, causing changes in the tertiary structure.
- It is commonly used in nucleic acid extraction as a cleaning solution (> 3중), binding solution (4-6중) for protein removal, and lysate for genomic DNA and total RNA extraction.
- Unlike guanidine isothiocyanate, guanidine hydrochloride exhibits low absorbance in A260, A280, and A230, resulting in an ideal ratio of nucleic acid product A260/A230.
명세서
| 특징 | 사양 | |
| 기본 콘텐츠 | 추천 애플리케이션 | Plasmid extraction, lysis Solution, or cleaning solution |
| CAS 번호 | 50-01-1 | |
| 공식 | CH5N3.HCL | |
| 분자 무게 | 95.53 | |
| 콘텐츠 | 99.5% | |
| 수준 | 분자 생물학 | |
| 모습 | White crystal will harden for a long time | |
| Transportation conditions | 실온 | |
| 보존 조건 | 실온, dry, dark | |
| 불순물 매개변수 | 수분 | ≤0.3% |
| Ash | ≤0.02% | |
| Water insoluble matter | ≤0.02% | |
| Ammonium | ≤0.05% | |
| Ammonium dicyanide | ≤0.1% | |
| Ammonium cyanuric chloride | ≤0.01% | |
| 철 | ≤0.1PPM | |
| UV 흡수 값 | 흡광도 값 @ 230 (6중) | ≤0.4 |
| 흡광도 값 @ 260 (6중) | ≤0.04 | |
| 흡광도 값 @ 280 (6중) | ≤0.01 | |
| 흡광도 값 @ 320 (6중) | ≤0.01 | |
| 핵산 추출 관련 | Plasmid extraction test | Adopt |
| Blood DNA extraction test | Adopt | |
| DNase test (2중) | 감지되지 않음 | |
| RNase test (2중) | 감지되지 않음 | |
| Purity of saturated solution (8중) | The 8m mother liquor is heated and dissolved. After dissolution, the insoluble matter is filtered with 3 layers of rapid filter paper. | |
| PH value (6중) | 5.0-5.5 |
주문정보
| 고양이.번호. | 상품명 | 패키지 |
| C11101 | Guanidine Hydrochloride (분자 생물학) | 5킬로그램 |
| C11102 | 25킬로그램 (5KG/Bag, 5Bags/Bucket) |
상품평
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