소개

HiPure Bacterial RNA Kit uses silica gel column purification to simplify the extraction. The whole process does not require phenol chloroform extraction and time-consuming alcohol precipitation. The kit is suitable for efficiently extracting RNA from various bacterial samples. The purified RNA can be directly used for RT-PCR, Northern hybridization, 등. The kit has included lysozyme and glass beads, which can be used to treat gram-negative bacteria which is easy to be lysed, as well as gram-positive bacteria which is hard to be lysed, including enterococcus faecalis, staphylococcus, 등.

세부

명세서

원칙

Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (~와 같은 구아니딘 hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. 마지막으로, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

장점

• 빠른 – 여러 샘플을 추출할 수 있습니다. 30 분
• 고순도 – the purified RNA can be directly used in various downstream applications
• 높은 회복력 – RNA can be recovered at the level of PG
• 좋은 반복성 – silica gel column purification technology can obtain ideal results every time
• Broad spectrum – it can deal with various bacteria, including Gram-positive bacteria that are difficult to be lysed
• Sufficient components – the kit contains carried lysozyme and glass beads

키트 내용물

보관 및 안정성

MagZol Reagent and Lysozyme should be stored at 2-8°C upon arrival. 나머지 키트 구성품은 실온에서 건조한 상태로 보관할 수 있습니다. (15-25℃) 그리고 적어도 안정적이다 18 이런 조건에서 몇 달 동안. 전체 키트는 2~8°C에서 보관할 수 있습니다., 그러나 이 경우 버퍼는 사용하기 전에 다시 용해되어야 합니다.. 사용 시 모든 버퍼가 실온에 있는지 확인하십시오..