소개
Blood is a valuable source of DNA for various clinical applications and research studies. 하지만, extracting DNA from blood samples presents several challenges:
Safety Concerns:
- Blood samples can harbor pathogens, posing a risk of infection to researchers and contaminating the lab environment.
- Traditional methods often involve hazardous chemicals like phenol-chloroform.
DNA Loss:
- Removing red blood cells, a common pre-treatment step can lead to the loss of desired DNA fractions like viral DNA, circulating DNA, and microbial DNA.
Impurities and Inhibitors:
- Blood contains various components that can interfere with downstream analyses like PCR 및 서던 블로팅.
New Technology for Improved Blood DNA Extraction:
This product offers a novel approach to address these challenges:
- Safe and Efficient: The method eliminates the need for red blood cell removal, minimizing infectious waste and potential contamination.
- Comprehensive DNA Recovery: The protocol allows for the purification of total DNA, including genomic, 미토콘드리아, 바이러스의, and circulating DNA, providing a more complete picture for analysis.
- 광범위한 샘플 호환성: The method effectively purifies DNA from various blood components like whole blood (fresh or frozen), 혈장, 혈청, 버피 코트, 그리고 더.
- 다운스트림 애플리케이션: The extracted high-quality DNA is suitable for reliable PCR and Southern blotting analyses.
This innovative solution simplifies blood DNA extraction while ensuring safety, 능률, and comprehensive DNA recovery. It represents a significant advancement for researchers working in various fields like diagnostics, 법의학, and medical research.
명세서
| 특징 | 명세서 |
| 주요 기능 | Isolation total DNA from 200ul Whole Blood |
| 응용 | PCR, Southern Bolt 및 바이러스 감지, 등 |
| 정제방법 | 미니 스핀 컬럼 |
| 정제기술 | 실리카 기술 |
| 가공방법 | 수동 (원심분리 또는 진공) |
| 샘플 유형 | Whole Blood (fresh or frozen), 혈청, 혈장, milk, 타액, and other liquid samples and cultured cells |
| 샘플량 | <200μl whole blood or other liquid samples, <5*106 lymphocytes or Culture Cells Non-mammalian animals that have a nucleus in red blood cells (rich in DNA, such as birds and fish): 5~20μl whole blood at a time. |
| 용출량 | ≥20μl |
| 실행당 시간 | 30분 이하 |
| 컬럼당 액체 운반량 | 800μl |
| 컬럼의 결합 수율 | 100µg |
원칙
This method provides a reliable and convenient solution for researchers working in various fields that require purified DNA for analysis, PCR과 같은, 서던 블로팅, 및 차세대 시퀀싱.
Efficient DNA Purification with Silica Column Technology
This product utilizes a silica column-based method for rapid and efficient DNA purification. Here’s a breakdown of the process:
- Sample Lysis and Digestion: The sample is treated with a lysate solution and protease to break down cell membranes and degrade proteins. This releases DNA into the solution.
- 실리카 막에 결합: The lysate containing the DNA is transferred to a silica column. The silica membrane in the column specifically binds DNA molecules through favorable interactions. Proteins and other impurities remain unbound.
- 세탁: 세척 버퍼가 컬럼을 통과합니다., removing unbound proteins and cellular debris that did not adhere to the silica membrane.
- 용출: 마지막으로, a low-salt buffer (일반적으로 10 mM 트리스, pH 9.0, 그리고 0.5 mM EDTA) 실리카 막에서 정제된 DNA를 용출하는 데 사용됩니다.. This buffer disrupts the interaction between DNA and the silica, 정제된 DNA를 별도의 튜브에 수집할 수 있음.
장점
- 고품질 DNA – 다양한 다운스트림 애플리케이션을 충족합니다., PCR을 포함하여, qPCR, 효소 소화, 이종 교잡, 등.
- 빠른 – 백혈구 분리 없이, 유기 추출, 또는 에탄올 침전
- 단순한 – 모든 핵산은 직접 소화로 얻을 수 있다
- 폭넓은 적용성- 다양한 액체 샘플 처리
키트 내용물
| 내용물 | D311102 | D311103 |
| 정화 시간 | 50 | 250 |
| HiPure DNA 미니 컬럼 I | 50 | 2 엑스 125 |
| 2ml 수집 튜브 | 100 | 5 엑스 100 |
| 버퍼 AL | 15 밀리리터 | 60 밀리리터 |
| Buffer DW1 | 30 밀리리터 | 150 밀리리터 |
| 버퍼 GW2* | 12 밀리리터 | 50 밀리리터 |
| 단백질분해효소 K | 24 mg | 120 mg |
| 프로테아제 용해 완충액 | 1.8 밀리리터 | 10 밀리리터 |
| 버퍼 AE | 15 밀리리터 | 60 밀리리터 |
보관 및 안정성
For optimal performance:
- Store Proteinase K at 2-8°C (refrigerator) upon arrival.
Acceptable short-term storage:
- Proteinase K can be stored for up to 12 weeks at room temperature (15-25℃) without affecting its performance.
Remaining kit components:
- Store all other components of the kit dry at room temperature (15-25℃).
- They are stable for at least 18 이런 조건에서 몇 달 동안.
Important Note:
- If storing the entire kit at room temperature, ensure buffers are redissolved before use and always warm all buffers to room temperature before using them.
