소개

The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples.

세부

명세서

원칙

Biological samples are first lysed and homogenized in a highly denaturing 구아니딘 isothiocyanate-containing buffer, which immediately inactivates DNases and RNases to ensure isolation of intact DNA and RNA. The lysate is then passed through a Mini spin column. This column, in combination with the high-salt buffer, allows selective and efficient binding of genomic DNA. Flow-through from the column is digested by Proteinase K in the presence of ethanol. This optimized digestion, together with the subsequent addition of further ethanol, allows appropriate binding of total RNA, including miRNA, to the column. Contaminants are efficiently washed away and high-quality RNA is eluted.

장점

• High quality – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
• 빠른 – 여러 샘플을 추출할 수 있습니다. 40 분
• High applicability – samples including animals, 식물, 박테리아, 세포, 등.
• 고농도 – efficiently remove macromolecular RNA, enrich small RNA and improve sensitivity
• Safe – no phenol chloroform extraction and no use of Trizol reagent

키트 내용물

보관 및 안정성

Proteinase K는 도착 시 2~8°C에서 보관해야 합니다.. 하지만, 단기 보관 (까지 12 주) 실온에서 (15-25℃) 성능에 영향을 미치지 않습니다. 나머지 키트 구성품은 실온에서 보관 가능 (15-25℃) 그리고 적어도 안정적이다 18 이런 조건에서 몇 달 동안.