HiPure DNA Clean Up Kit usesproprietary chemistry and HiPure technology to recover DNA Fragments between20bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, 시퀀싱,restriction digestion, or various labeling reactions. 게다가, this kit canbe also used to recover DNA directly from enzymatic reactions such as PCR andenzyme digestion reactions.
세부
명세서
| 특징 | 명세서 |
| 주요 기능 | Recover DNA fragments between 20bp-20kbp from crude DNA and enzymatic reaction solution |
| 응용 | PCR, 시퀀싱, labeling reactions, ligations and restriction digestion, 등. |
| 정제방법 | 미니 스핀 컬럼 |
| 정제기술 | 실리카 기술 |
| 가공방법 | 수동 (원심분리 또는 진공) |
| 샘플 유형 | Crude DNA, enzymatic reaction solution |
| 샘플량 | <span “=”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”><150μl |
| 회복 | 80% |
| 용출량 | <span “=”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>≥15μl |
| 실행당 시간 | <span “=”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>≤10 minutes |
| 컬럼당 액체 운반량 | 800μl |
| 컬럼의 결합 수율 | 20µg |
원칙
The HiPure system uses a simple bind-wash-elute procedure. Gelslices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
장점
- High recovery efficiency – 까지 80% DNA recovery
- 빠른 – isolation can be completed in 10 minutes by column gel method
- Low elution volume – elution volume can be as low as 10μl
- Efficient – efficient removal of various inhibitory factors
키트 내용물
| 내용물 | D214102 | D214103 |
| 정화 시간 | 100 준비 | 250 준비 |
| Buffer GWP | 30 밀리리터 | 60 밀리리터 |
| Buffer DW2 | 20 밀리리터 | 50 밀리리터 |
| 용출 버퍼 | 30 밀리리터 | 60 밀리리터 |
| HiPure DNA 미니 컬럼 I | 50 | 250 |
| 2 ml 수집 튜브 | 50 | 250 |
보관 및 안정성
HiPure DNA Clean Up Kit components are guaranteed for at least one year when stored at room temperature. If any precipitates form in the buffers, warm at 37℃ to dissolve.
상품평
아직 상품평이 없습니다.