HiPure FFPE DNA 키트

The HiPure FFPE Nucleic Acid Kit offers a fast and user-friendly solution for extracting DNA from formalin-fixed, 파라핀이 내장된 (FFPE) tissue samples.

• Target Samples: FFPE tissue and sections
• Simple and Rapid: DNA extraction can be completed in just 20 분 (excluding digestion time).
• Silica Gel Technology: Employs a reliable silica gel column purification method, eliminating the need for harsh organic solvents (phenol-chloroform) or alcohol precipitation steps.
• 다운스트림 애플리케이션: Purified DNA is suitable for various analyses like PCR (폴리 메라 제 연쇠 반응), 서던 블로팅, and viral DNA detection.

이익:

• 간소화된 작업 흐름: The silica gel column technology offers a faster and less hazardous alternative to traditional methods.
• High-Quality DNA: The extraction process ensures the isolation of DNA suitable for diverse downstream applications.
• Broad Applicability: The kit works effectively with various FFPE tissue samples.

This kit empowers researchers working in various fields, 종양학을 포함한, pathology, and infectious diseases, to efficiently extract DNA from archived FFPE samples for further analysis.

명세서

원칙

HiPure FFPE Nucleic Acid Kit, designed for rapid and efficient extraction of both DNA and RNA from formalin-fixed, 파라핀이 내장된 (FFPE) tissue samples:

주요 특징들:

• Silica Gel Column Technology: Utilizes a reliable and streamlined approach, eliminating the need for harsh chemicals like phenol-chloroform.
• Fast Processing: Extracts DNA/RNA in just 20 분, excluding digestion time.
• 샘플 호환성: Works effectively with various FFPE tissue sections.

추출과정:

1. Deparaffinization: Xylene removes paraffin wax, a common component used to preserve tissues.
2. 용해 및 소화: A special solution (용해하다) 그리고 효소 (프로테아제) break down cells and proteins, releasing nucleic acids (DNA and RNA) into the solution.
3. De-Crosslinking: Heating at 90°C reverses the effects of formalin fixation, allowing easier access to nucleic acids.
4. 실리카 막에 결합: After adjusting conditions with ethanol, the sample is transferred to a column. DNA/RNA binds to the silica membrane, while proteins pass through.
5. 세탁: Buffers remove impurities like proteins and salts from the captured nucleic acids.
6. 용출: A low-salt buffer elutes the purified DNA/RNA, making it ready for downstream applications.

응용:

• PCR (폴리 메라 제 연쇠 반응)
• 서던 블로팅
• Viral nucleic acid detection
• Other analyses requiring high-quality DNA or RNA

장점

The HiPure FFPE Nucleic Acid Kit offers a superior solution for extracting DNA and RNA from formalin-fixed, 파라핀이 내장된 (FFPE) tissue samples.

Safety:

• No Xylene Contact: Eliminates the need for hazardous xylene, minimizing exposure to harmful chemicals.

속도:

• Fast Processing: Extracts DNA/RNA in just 20 분 (excluding digestion time), allowing you to process multiple samples within 2 시간.
• No Overnight Incubation: Streamlines the workflow by eliminating the need for lengthy overnight incubation steps.

고효율:

• Enhanced PCR Sensitivity: Effectively removes formaldehyde modifications from DNA, significantly improving the sensitivity of downstream PCR (폴리 메라 제 연쇠 반응) 복수.

키트 내용물

보관 및 안정성

• 단백질분해효소 K: Keep it cool in the refrigerator (2-8℃) 최대 효과를 위해 도착 시.
• Short-Term Storage (까지 12 주): Proteinase K can last at room temperature (15-25℃) 최대 12 문제 없이 몇 주.
• Everything Else: Store other kit components at room temperature (15-25℃). They are stable for at least 18 이런 조건에서 몇 달 동안.
• 중요한: If storing the entire kit cold, 사용하기 전에 버퍼를 예열하는 것을 잊지 마세요. All buffers should be at room temperature for optimal results.

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